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From 2013.igem.org

Contents 1 Laborplan Pre-project 2 Motivation for Proposal (discussed with Tim & Tinka) 3 3. Podiums discussion 27.6.13 3.1 Cloning

Laborplan Pre-project

• prepare ACM (1,5L + 0,5L (mit Agar)) Phytogel(NOT with Agar)
• reactivation solution (without agar)
• 0,5% agarose solution with ACM (maybe water) with 10mM AuCl3
• purification
  • HP 20 resin-beads: the Delftibactin attaches to the resin
  • Methanol dissolves the Delftibactin, afterwards dry (rotary vacuum)
  • resuspend it (in 2ml)

Motivation for Proposal (discussed with Tim & Tinka)

• central idea: NRPS (gold is the application)
• Weighing not clear
• start with gold
• Structure: Application = Framework
• Main part: relevance of NRPS
• generally more elaborate (not too much details)
• more numbers (tons of gld recycled)
• more structure
• why this pathway for gold precipitation and not another one
• don't use might or may
• softwareproject: how important is it for the gold?? why is it important??? judgement
• Trends of IGEM: no NRPS so far, thus new project for IGEM -> helps students and scientist in finding trends, etc...

3. Podiums discussion 27.6.13

• 18.00 talk about synthetic biology
• 18.30 discussion
• church, politics, scientists, NGOs(?) - 5 people
• Prof. Tanner
• first of all: make a concept with our goal
• catering afterwards (sponsoring)

Cloning

• BACs
• amplify genes via PCR (Problem, same Codons for E.coli and D.acidovorans)
• maybe not optimal codons
• Synthesis is not possible
• Biobrickstandard: separation in 1 Kb sequenzes
• first plasmids with one protein each
• then plasmids with multiple proteins
• and another try with whole pathway (BAC)
• now we need to find experts and ask them for help