Team:Paris Bettencourt/Notebook/Week4

From 2013.igem.org

Contents

Week 4

Monday 24th June

Tuesday 25th June

Wednesday 26th June

Thursday 27th June

Friday 28th June

Trojan Horse

Edit/Create this note

sRNA design

sRNA Design

Red sequences:

pACYCDuet-1 (CmR) : ATATCCAGTGATTTTTTTCTCCAT
pCOLADuet-1 (KanR) : CGTTTCCCGTTGAATATGGCTCAT

We decided that targeting only two different antibiotic resistance will be enough for a proof of concept. We give up AmpR because otherwise they might be experimental issues with AmpR that is also on phagemid litmus28i.

Saturday 29th June

Sunday 30th June