"
Team:Utah State/Results
From 2013.igem.org
Skiracer96 (Talk | contribs) |
Skiracer96 (Talk | contribs) |
||
| Line 20: | Line 20: | ||
<div style="display: inline-block; position: absolute; top: 160px; margin-left: 250px; width: 500px;"> | <div style="display: inline-block; position: absolute; top: 160px; margin-left: 250px; width: 500px;"> | ||
<div> | <div> | ||
| - | <img src="https://static.igem.org/mediawiki/igem.org/5/5f/USUResults.png" | + | <img src="https://static.igem.org/mediawiki/igem.org/5/5f/USUResults.png"> |
</div> | </div> | ||
<br> | <br> | ||
| Line 50: | Line 50: | ||
</p> | </p> | ||
</div> | </div> | ||
| - | <img src="https://static.igem.org/mediawiki/2013/1/10/AtgHTGFP.png"> | + |   |
| + |   | ||
| + |   | ||
| + |   | ||
| + |   | ||
| + |   | ||
| + |   | ||
| + |   <img src="https://static.igem.org/mediawiki/2013/1/10/AtgHTGFP.png" alt="GFP" width="120" height="300"> | ||
<a name="Results"><div class="Header1"> | <a name="Results"><div class="Header1"> | ||
Revision as of 21:50, 24 September 2013
Text descriptions go here.
Text descriptions go here.
To demonstrate that the N-terminal 10x His Tag (BBa_K1162009) functions correctly, it was cloned in front of Green Florescent Protein (GFP)with the lac promoter+rbs system (BBa_K208010)to give the complete construct BBa_K1162013. This construct was expressed in E. coli grown in 50mL LB media with the addition of chloramphenicol and induced with IPTG. After allowing to grow overnight the cells were spun down and protein was purified with a nickel spin column (see protein purification protocol on protocols page). Fractions from this nickel spin column procedure were saved and run on an SDS-PAGE gel (see below). Since this was a GFP purification procedure, the different fractions were dotted on parafilm and place on a UV gel box to visualize the protein (see below).
Text descriptions go here.
Text descriptions go here.
Text descriptions go here.
