Team:Paris Bettencourt/Notebook/Trojan Horse/Friday 23rd August.html

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<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Trojan_Horse" target="_blank" class="tbnotelogo THlogo"> ASDF </a>
<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Trojan_Horse" target="_blank" class="tbnotelogo THlogo"> ASDF </a>
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<h3>23<sup>rd</sup> August</h3>
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<h3>24<sup>th</sup> August</h3>
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Revision as of 17:59, 3 October 2013

Trojan Horse

24th August


Results: failure: as much GFP+ colonies growing on Kan than on LB

PCR from mini Gene sRNA anti Cm

35 H2O

10 Buffer 5x

1 dNTP

2 FW iT009

2 RV iT0010

0.2 minigene-plasmid amp from IDT

0.5 phusion

PCR Phusion Gradient around 53 °C

Time : 30 secondes