Team:SDU-Denmark/Tour44

From 2013.igem.org

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<h2>SOPs</h2>
<h2>SOPs</h2>
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<h4>Standard operating procedures</h4>
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<h4>A complete anthology of good routines</h4>
<p class='intro'>
<p class='intro'>
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“Ones more unto the breach, dear friends, once more” - Henry V
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“Once more unto the breach, dear friends, once more” - <b>Henry V</b>
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</p>
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We used an arsenal of self-written Standard Operating Procedures (SOPs) to execute the vast amount of laboratory work. Each was written in collaboration with a person with experience in the field of the procedure and then approved by a second competent person. As such, it was strictly forbidden to change the SOPs without consulting the rest of the team, and any changes were then implemented in agreement. It was hereby secured that everyone in the lab were following the same procedures each time.  
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<span class="intro">We used an arsenal</span> of self-written Standard Operating Procedures (SOPs) to execute the vast amount of laboratory work. Each was written in collaboration with a person with experience in the field of the procedure and then approved by a second competent person. As such, it was strictly forbidden to change the SOPs without consulting the rest of the team, and any changes were then implemented in agreement. It was hereby secured that everyone in the lab were following the same procedures each time.  
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</p>
<p>
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<b>List of the different SOPs;</b> Each name is a hyperlink connected to an online PDF of the SOP.
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Below you can explore all our different SOPs  
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<div class="accordion alignCenter" style="width:500px;">
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  <div class="accordionTitel">First pane</div>
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  <div class="pane current" style="height:300px;" >
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/ef/SDU2013_SOP0001_v01.pdf" target="_blank">iGEM2013_SOP0001_v01_MM_ON_culture_of_E.coli</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/ef/SDU2013_SOP0001_v01.pdf" target="_blank">iGEM2013 SOP0001 v01 - ON culture of <span class="specialWord">E. coli</span></a><br>
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<a class="pdfFile"href="https://static.igem.org/mediawiki/2013/5/5b/SDU2013_SOP0002_v01.pdf" target="_blank">iGEM2013_SOP0002_v01_TK_Table_autoclave</a><br>
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<a class="pdfFile"href="https://static.igem.org/mediawiki/2013/5/5b/SDU2013_SOP0002_v01.pdf" target="_blank">iGEM2013 SOP0002 v01 - Table autoclave</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/88/SDU2013_SOP0003_v01.pdf" target="_blank">iGEM2013_SOP0003_v01_MM_Bacterial_freezing_stock</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/88/SDU2013_SOP0003_v01.pdf" target="_blank">iGEM2013 SOP0003 v01 - Bacterial freezing stock</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/5/59/SDU2013_SOP0004_v01.pdf" target="_blank">iGEM2013_SOP0004_v01_Primer_Design</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/5/59/SDU2013_SOP0004_v01.pdf" target="_blank">iGEM2013 SOP0004 v01 - Primer Design</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/f/f2/SDU2013_SOP0005_v01.pdf" target="_blank">iGEM2013_SOP0005_v01_USER primer design</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/f/f2/SDU2013_SOP0005_v01.pdf" target="_blank">iGEM2013 SOP0005 v01 - USER primer design</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/73/SDU2013_SOP0006_v01.pdf" target="_blank">iGEM2013_SOP0006_v01_PCR protocol for USER cloning</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/73/SDU2013_SOP0006_v01.pdf" target="_blank">iGEM2013 SOP0006 v01 - PCR protocol for USER cloning</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/a/a1/SDU2013_SOP0007_v01.pdf" target="_blank">iGEM2013_SOP0007_v01_excision and ligation of PCR product in USER cloning</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/a/a1/SDU2013_SOP0007_v01.pdf" target="_blank">iGEM2013 SOP0007 v01  - excision and ligation of PCR product in USER cloning</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/6/6e/SDU2013_SOP0008_v01.pdf" target="_blank">iGEM2013_SOP0008_v01_preparing primers</a>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/6/6e/SDU2013_SOP0008_v01.pdf" target="_blank">iGEM2013 SOP0008 v01 - preparing primers</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/71/SDU2013_SOP0009_v01.pdf" target="_blank">iGEM2013 SOP0009 v01 - TSB transformation</a><br>
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  <div class="accordionTitel">Second pane</div>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/9/93/SDU2013_SOP0010_v01.pdf" target="_blank">iGEM2013 SOP0010 v01 - Phusion PCR</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/b/b6/SDU2013_SOP0011_v01.pdf" target="_blank">iGEM2013 SOP0011 v01 - MyTaq PCR</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/71/SDU2013_SOP0009_v01.pdf" target="_blank">iGEM2013_SOP0009_v01_TSB transformation</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/74/SDU2013_SOP0012_v01.pdf" target="_blank">iGEM2013 SOP0012 v01  - Restriction Digest</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/9/93/SDU2013_SOP0010_v01.pdf" target="_blank">iGEM2013_SOP0010_v01_Phusion PCR</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/c/cd/SDU2013_SOP0013_v01.pdf" target="_blank">iGEM2013 SOP0013 v01 - Nano-drop</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/b/b6/SDU2013_SOP0011_v01.pdf" target="_blank">iGEM2013_SOP0011_v01_PCR with MyTaq</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_SOP0014_v01.pdf" target="_blank">iGEM2013 SOP0014 v01 - Gel purification</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/7/74/SDU2013_SOP0012_v01.pdf" target="_blank">iGEM2013_SOP0012_v01_Restriction_Digest</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/6/64/SDU2013_SOP0015_v01.pdf" target="_blank">iGEM2013 SOP0015 v01 - Ligation</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/c/cd/SDU2013_SOP0013_v01.pdf" target="_blank">iGEM2013_SOP0013_v01_Nano-drop</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/2/2c/SDU2013_SOP0016_v01.pdf" target="_blank">iGEM2013 SOP0016 v01 - LB and LA media</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/a/a8/SDU2013_SOP0014_v01.pdf" target="_blank">iGEM2013_SOP0014_v01_Gel purification</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/86/SDU2013_SOP0017_v01.pdf" target="_blank">iGEM2013 SOP0017 v01 - Fast digest</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/6/64/SDU2013_SOP0015_v01.pdf" target="_blank">iGEM2013_SOP0015_v01_Ligation</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/ed/SDU2013_SOP0018_v01.pdf" target="_blank">iGEM2013 SOP0018 v01 - Agar plates with Cm</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/2/2c/SDU2013_SOP0016_v01.pdf" target="_blank">iGEM2013_SOP0016_v01_Making LB and LA media</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/85/SDU2013_SOP0019_v01.pdf" target="_blank">iGEM2013 SOP0019 v01 - Plasmid Miniprep</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/86/SDU2013_SOP0017_v01.pdf" target="_blank">iGEM2013_SOP0017_v01_Fast_digest</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/b/b2/SDU2013_SOP0020_v01.pdf" target="_blank">iGEM2013 SOP0020 v01 - Agarose gel DNA</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/ed/SDU2013_SOP0018_v01.pdf" target="_blank">iGEM2013_SOP0018_v01_Agar plates with Cm</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/1/15/SDU2013_SOP0021_v01.pdf" target="_blank">iGEM2013 SOP0021 v01 - MyTaq Colony PCR</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/8/85/SDU2013_SOP0019_v01.pdf" target="_blank">iGEM2013_SOP0019_v01_Plasmid Miniprep</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/5/56/SDU2013_SOP0022_v01.pdf" target="_blank">iGEM2013 SOP0022 v01 - Speedy Vac</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/b/b2/SDU2013_SOP0020_v01.pdf" target="_blank">iGEM2013_SOP0020_v01_MM_Agarose_gel_DNA</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/2/2b/SDU2013_SOP0023_v01.pdf" target="_blank">iGEM2013 SOP0023 v01 - PCR reactions for Site directed mutagenesis of Dxs from B. subtilis</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/1/15/SDU2013_SOP0021_v01.pdf" target="_blank">iGEM2013_SOP0021_v01_TK_Colony_PCR_with_MyTaq</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/4/4e/SDU2013_SOP0024_v01.pdf" target="_blank">iGEM2013 SOP0024 v01 - Growth experiment</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/5/56/SDU2013_SOP0022_v01.pdf" target="_blank">iGEM2013_SOP0022_v01_Speedy Vac</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/e9/SDU2013_SOP0025_v01.pdf" target="_blank">iGEM2013 SOP0025 v01 - Growth for RNA purification - IPTG inducable plasmid</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/2/2b/SDU2013_SOP0023_v01.pdf" target="_blank">iGEM2013_SOP0023_v01_SDM_ PCR reactions for Site directed mutagenesis of Dxs from B. subtilis</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/c/cf/SDU2013_SOP0026_v01.pdf" target="_blank">iGEM2013 SOP0026 v01 - Growth for RNA purification - ara inducible plasmid</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/4/4e/SDU2013_SOP0024_v01.pdf" target="_blank">iGEM2013_SOP0024_v01_Growth_experiment</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/1/1a/SDU2013_SOP0027_v01.pdf" target="_blank">iGEM2013 SOP0027 v01 - RNA purification</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/e/e9/SDU2013_SOP0025_v01.pdf" target="_blank">iGEM2013_SOP0025_v01_Growth for RNA purification - IPTG inducable plasmid</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/b/bc/SDU2013_SOP0028_v01.pdf" target="_blank">iGEM2013 SOP0028 v01 - Northen blotting</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/c/cf/SDU2013_SOP0026_v01.pdf" target="_blank">iGEM2013_SOP0026_v01_Growth for RNA purification - ara inducible plasmid</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/9/98/SDU2013_SOP0029_v01.pdf" target="_blank">iGEM2013 SOP0029 v01 - Kinating probe for northern blot</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/1/1a/SDU2013_SOP0027_v01.pdf" target="_blank">iGEM2013_SOP0027_v01_RNA purification</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/4/48/IGEM2013_SOP0030_v01.pdf" target="_blank">iGEM2013 SOP0030 v01 - Rubber purification</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/b/bc/SDU2013_SOP0028_v01.pdf" target="_blank">iGEM2013_SOP0028_v01_Northen blotting</a><br>
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<a class="pdfFile" href="https://static.igem.org/mediawiki/2013/9/98/SDU2013_SOP0029_v01.pdf" target="_blank">iGEM2013_SOP0029_v01_Kinating probe for northern blot</a><br>
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  </div>
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Latest revision as of 22:55, 28 October 2013

Bacteriorganic Rubber
iGEM logo
Introduction
Rubber issue
Production system
Process
Results
Future

SOPs

A complete anthology of good routines

“Once more unto the breach, dear friends, once more” - Henry V

We used an arsenal of self-written Standard Operating Procedures (SOPs) to execute the vast amount of laboratory work. Each was written in collaboration with a person with experience in the field of the procedure and then approved by a second competent person. As such, it was strictly forbidden to change the SOPs without consulting the rest of the team, and any changes were then implemented in agreement. It was hereby secured that everyone in the lab were following the same procedures each time.

Below you can explore all our different SOPs

iGEM2013 SOP0001 v01 - ON culture of E. coli
iGEM2013 SOP0002 v01 - Table autoclave
iGEM2013 SOP0003 v01 - Bacterial freezing stock
iGEM2013 SOP0004 v01 - Primer Design
iGEM2013 SOP0005 v01 - USER primer design
iGEM2013 SOP0006 v01 - PCR protocol for USER cloning
iGEM2013 SOP0007 v01 - excision and ligation of PCR product in USER cloning
iGEM2013 SOP0008 v01 - preparing primers
iGEM2013 SOP0009 v01 - TSB transformation
iGEM2013 SOP0010 v01 - Phusion PCR
iGEM2013 SOP0011 v01 - MyTaq PCR
iGEM2013 SOP0012 v01 - Restriction Digest
iGEM2013 SOP0013 v01 - Nano-drop
iGEM2013 SOP0014 v01 - Gel purification
iGEM2013 SOP0015 v01 - Ligation
iGEM2013 SOP0016 v01 - LB and LA media
iGEM2013 SOP0017 v01 - Fast digest
iGEM2013 SOP0018 v01 - Agar plates with Cm
iGEM2013 SOP0019 v01 - Plasmid Miniprep
iGEM2013 SOP0020 v01 - Agarose gel DNA
iGEM2013 SOP0021 v01 - MyTaq Colony PCR
iGEM2013 SOP0022 v01 - Speedy Vac
iGEM2013 SOP0023 v01 - PCR reactions for Site directed mutagenesis of Dxs from B. subtilis
iGEM2013 SOP0024 v01 - Growth experiment
iGEM2013 SOP0025 v01 - Growth for RNA purification - IPTG inducable plasmid
iGEM2013 SOP0026 v01 - Growth for RNA purification - ara inducible plasmid
iGEM2013 SOP0027 v01 - RNA purification
iGEM2013 SOP0028 v01 - Northen blotting
iGEM2013 SOP0029 v01 - Kinating probe for northern blot
iGEM2013 SOP0030 v01 - Rubber purification

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