Team:MIT/ResultsBiobricks

From 2013.igem.org

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<li>Length: 2085
<li>Length: 2085
<li>Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.<br><br>
<li>Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.<br><br>
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Other parts we like:
 
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<table border="1">
 
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<tr>
 
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<td>Acyl-TyA</td>      <td>BBa_K1179000</td>
 
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</tr><tr>
 
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<td>Acyl-TyA-eGFP</td> <td>BBa_K1179001</td>
 
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</tr><tr>
 
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<td>Hef1a_Cas9-VP16</td> <td>BBa_K1179002</td>
 
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</tr><tr>
 
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<td>Acyl-TyA-mKate-His</td> <td>BBa_K1179003</td>
 
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</tr><tr>
 
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<td>Acyl-TyA-rtTA</td> <td>BBa_K1179004</td>
 
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</tr><tr>
 
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<td>Acyl-TyA-rtTA-His</td> <td>BBa_K1179005</td>
 
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</tr><tr>
 
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<td>Acyl-TyA-Tev</td> <td>BBa_K1179006</td>
 
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</tr><tr>
 
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<td>Acyl-TyA-TEV-rtTA-His</td> <td>BBa_K1179007</td>
 
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</tr>
 
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Cas9 D10A & H840A mutation-linker <td>BBa_K1179008
 
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Cas9-Linker-VP16 BBa_K1179009
 
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eBFP BBa_K1179010
 
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eYFP BBa_K1179012
 
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eYFP-PEST BBa_K1179013
 
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VP16-GAL4 BBa_K1179014
 
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U6_gRNA (Cr9) BBa_K1179015
 
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L7Ae BBa_K1179016
 
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Linker-Cas9 D10A & H840A mutation BBa_K1179017
 
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mKate –PEST BBa_K1179018
 
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mKate-His BBa_K1179019
 
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rtTA-His BBa_K1179020
 
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CAG_Acyl-TyA-mKate-His BBa_K1179021
 
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CAG_Acyl-TyA-rtTA BBa_K1179022
 
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CAG_Acyl-TyA-rtTA-His BBa_K1179023
 
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CAG_eYFP-PEST BBa_K1179024
 
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CAG_L7Ae BBa_K1179025
 
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CAG_mKate-PEST BBa_K1179026
 
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CAG-tagBFP BBa_K1179027
 
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D10A and H840A mutant Cas9-VC155 BBa_K1179028
 
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D10A and H840A mutant Cas9-VN155 BBa_K1179029
 
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D10A and H840A mutant Cas9-VN173 BBa_K1179030
 
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CMV_ L7Ae_myc-His6 BBa_K1179032
 
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CMV_2xKMet-eGFP BBa_K1179033
 
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L7Ae-2a-eYFP BBa_K1179037
 
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TRE_Cas9-VP16 BBa_K1179040
 
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U6_gRNA 1A Reverse BBa_K1179041
 
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U6_gRNA 1A BBa_K1179042
 
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U6_gRNA 2A BBa_K1179043
 
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U6_gRNA 3A BBa_K1179044
 
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U6_gRNA 4A BBa_K1179045
 
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U6_gRNA 5A BBa_K1179046
 
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U6_gRNA 6A BBa_K1179047
 
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U6_gRNA 6A Reverse BBa_K1179048
 
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VC155 BBa_K1179049
 
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VC155 with Leucine zipper BBa_K1179050
 
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VC155-Cas9(D10A and H840A mutant) BBa_K1179051
 
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VN155 BBa_K1179052
 
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VN155 with Leucine zipper BBa_K1179053
 
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VN155-Cas9(D10A and H840A mutant) BBa_K1179054
 
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VN173 BBa_K1179055
 
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VN173 with Leucine zipper BBa_K1179056
 
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VN173-Cas9(D10A and H840A mutant) BBa_K1179057
 
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Cre entry vector BBa_K1179058
 
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eYFP-4x451ts BBa_K1179060
 
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eYFP-4x503ts BBa_K1179061
 
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LacI BBa_K1179062
 
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LacI-4x451ts BBa_K1179063
 
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LacI-4x503ts BBa_K1179064
 
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TagBFP BBa_K1179068
 
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CAG BBa_K1179069
 
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CMV_Acyl-TyA-eGFP BBa_K1179071
 
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Hef1a_eYFP-4x451 BBa_K1179072
 
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TRE-tight_eYFP-4x451 BBa_K1179073
 
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Hef1a_eYFP-4x503 BBa_K1179074
 
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TRE-t_eYFP-4x503 BBa_K1179075
 
<div id="block-content">
<div id="block-content">

Revision as of 03:40, 28 September 2013

iGEM 2012

Overview

  • Project Overview

miRNA Signal

  • Overview
  • siRNA Characterization
  • Exosome Isolation and Co-Culturing
  • Cell-Cell Co-Culturing

Protein Signals

  • Overview
  • GFP
  • rtTA3
  • Cre
  • L7Ae
  • Cas9-VP16

Novel DNA Sensor: Cas9 Split Venus Fusion

  • Overview
  • Leucine Zipper Fusion
  • DNA Sensing

Our BioBricks

  • Favorites
  • All BioBricks

Attributions

  • Attributions

Biobricks


Favorite Parts:

Name: CMV_Acyl-TyA-eGFP MammoBlock
  • ID: BBa_K1179071
  • Type: Generator
  • Length: 2834
  • Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence.

    Name: Hef1a_Cas9-VP16 MammoBlock
  • ID: BBa_K1179002
  • Type: Generator
  • Length: 5141
  • Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with Cas9 binding sites upstream.

    Name: Hef1a_eYFP-4x451 MammoBlock
  • ID: BBa_K1179072
  • Type: Generator
  • Length: 2085
  • Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.