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Team:MSOE Milwaukee/Primers
From 2013.igem.org
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<TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase forward</TD> | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase forward</TD> | ||
| - | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC</TD> | + | <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
Revision as of 18:38, 8 July 2013
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| DNA SEQUENCE/ENZYME | PRIMER TO ADD THE PREFIX (FOR) 5' to 3' PRIMER TO ADD THE SUFFIX (REV) 5' to 3' |
| 1,8-cineole synthase forward | ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG |
| 1,8-cineole synthase reverse | GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG |
| mvaS-F | ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC |
| mvaS-R | GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG |
| mvaK2 | Phosphomevalonate kinase |
| mvaD | Mevalonate-5-pyrophosphate decarboxylase |
| TPS-CIN | 1,8-cineole synthase |
