Team:Heidelberg/Delftibactin/DelRest

From 2013.igem.org

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                                   <h1>Week 13</h1>
                                   <h1>Week 13</h1>
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In the previous week several of the new primers improved our PCRs for DelO-P. Nevertheless results were not convincing enough to use the amplified fragments for Gibson Assembly. The same encounts for the amplification of DelF-G. Here various strategies were conducted. Though none of these led to an entirely sucessful amplification of the genes DelF and DelG in this week
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In the previous week several of the new primers improved our PCRs for DelO-P. Nevertheless results were not convincing enough to use the amplified fragments for Gibson Assembly. The same encounts for the amplification of DelF-G. Here various strategies where conducted. Though none of these led to an entirely satisfying ampl of the genes DelF and DelG, we were able to amplify regions of the Delftibactin cluster which we could not amplifý with the initial primers. Therefore this week will be used for optimization of these PCRs as well as for validation of the amplicons we were already able to obtain in well established amplifications. Validation will be carried out with various restriction digests. PCR products showing positive digests will be prepared for single read sequencing to validate constructs before Gibson Assembly.
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Revision as of 13:06, 2 October 2013

Del Rest. Creating a 32 kbp plasmid.

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