Team:UC Chile/Overview

From 2013.igem.org

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                <h1>Overview</h1>
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    <h1>Overview</h1>
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                    We are working towards reengineering the Carboxysome, a bacterial microcompartment (BMC), to produce a genetically encoded platform for optimized packaging of metabolic processes and/or enclosed protein production systems.<br>
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In order to do so, we are developing new biobricks for targeting any protein of interest to the inside of Carboxysomes. We are estimating the structure and volume of the Carboxysome to assess and maximize production yields. Moreover, we are developing a novel bacterial microcompartment isolation method by adapting existing technologies to easily purify reengineered Carboxysomes while maintaining their structural and functional properties.<br>
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Imagine a world where production of different compound could be easily achievable, where industrial problems associated with genetic manipulation are over: there is no more instability in the energy flux of bacteria. Imagine a world where there are no more industrial biosafety issues because recombinant bacteria is no longer needed in situ. This is the world we propose: the world of in vitro production.
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Our project should facilitate novel applications using cellular or acellular systems for the compartmentalization of metabolic engineering reactions as well as providing a toolbox for safe and efficient production of biomolecules in bacterial systems.
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To achieve this, we reengineered the Carboxysome, a bacterial microcompartment (BMC) to use it as a continuous reactor capable of generating any output by having the needed enzyme in its inner space. Make Carboxysome your own synthetic space for the production of anything you want:
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A Whateversisome!
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We have successfully:  
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    <li>Characterized the Carboxysome as a tool for Synthetic Biology: we presented the plasmid as a standardized biobrick.</li>
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    <li>Found a targeting sequence to the inside of Carboxysome.</li>
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    <li>Designed an innovative methodology for purification and isolation of BMC.</li>
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    <li>SOMETHING ABOUT MODELING.</li>
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Even more…
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    <li> We are working towards generating step-by-step in vitro reactions.</li>
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Revision as of 14:21, 27 September 2013

Wiki-IGEM

Overview

Imagine a world where production of different compound could be easily achievable, where industrial problems associated with genetic manipulation are over: there is no more instability in the energy flux of bacteria. Imagine a world where there are no more industrial biosafety issues because recombinant bacteria is no longer needed in situ. This is the world we propose: the world of in vitro production. To achieve this, we reengineered the Carboxysome, a bacterial microcompartment (BMC) to use it as a continuous reactor capable of generating any output by having the needed enzyme in its inner space. Make Carboxysome your own synthetic space for the production of anything you want: A Whateversisome!
We have successfully:
  • Characterized the Carboxysome as a tool for Synthetic Biology: we presented the plasmid as a standardized biobrick.
  • Found a targeting sequence to the inside of Carboxysome.
  • Designed an innovative methodology for purification and isolation of BMC.
  • SOMETHING ABOUT MODELING.

Even more…
  • We are working towards generating step-by-step in vitro reactions.

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