Team:Cornell/project/wetlab/fungal toolkit/homologous constructs
From 2013.igem.org
(Difference between revisions)
Line 23: | Line 23: | ||
<div class="eight columns"> | <div class="eight columns"> | ||
<h3>Method</h3> | <h3>Method</h3> | ||
- | Constructs were made containing selected genes flanked by regions homologous to the <i>Ganoderma lucidum</i> gpd promoter. Thus, the construct would be compatible with homologous recombination. The construct was made using a three part gibson assembly. The three parts were all made in separate BioBricks containing the left and right homology regions and the selected genes. The genes inserted into the homology flanking regions include prtpc promoter, gpd promoter, T7 promoter, and the resistance genes hph and nptII. The constructs were then inserted into the respective fungus strain using protoplasting and PEG transformation methods [1]. | + | Constructs were made containing selected genes flanked by regions homologous to the <i>Ganoderma lucidum</i> gpd promoter. Thus, the construct would be compatible with homologous recombination. The construct was made using a three part gibson assembly. The three parts were all made in separate BioBricks containing the left and right homology regions and the selected genes. The genes inserted into the homology flanking regions include prtpc promoter, gpd promoter, T7 promoter, and the resistance genes hph and nptII. The constructs were then inserted into the respective fungus strain using protoplasting and PEG transformation methods [1]. |
</div> | </div> | ||
<div class="four columns"> | <div class="four columns"> |
Revision as of 02:38, 28 September 2013