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19/08/13
From 2013.igem.org
(Difference between revisions)
(→Running both denatured and sonicated samples on gel) |
(→Running both denatured and sonicated samples on gel) |
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*For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA. | *For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA. | ||
*5ul of 1kb ladder was used for 3 wells each. | *5ul of 1kb ladder was used for 3 wells each. | ||
| + | [[File:igem_denatured_son_190813.jpg]] | ||
Revision as of 13:57, 19 August 2013
Denaturing sonicated DNA
- Denaturing DNA by heat shock for 10mins in a 95C heat block
- Adding 5ul of OG dye
Running both denatured and sonicated samples on gel
- For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA.
- 5ul of 1kb ladder was used for 3 wells each.
