Team:MIT/ResultsBiobricks

From 2013.igem.org

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iGEM 2012

Overview

  • Project Overview

miRNA Signal

  • Overview
  • siRNA Characterization
  • Exosome Isolation and Co-Culturing
  • Cell-Cell Co-Culturing

Protein Signals

  • Overview
  • GFP
  • rtTA3
  • Cre
  • L7Ae
  • Cas9-VP16

Novel DNA Sensor: Cas9 Split Venus Fusion

  • Overview
  • Leucine Zipper Fusion
  • DNA Sensing

Our BioBricks

  • Favorites
  • All BioBricks

Attributions

  • Attributions

Biobricks


Favorite Parts:

Name: CMV_Acyl-TyA-eGFP MammoBlock
  • ID: BBa_K1179071
  • Type: Generator
  • Length: 2834
  • Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence.

    Name: Hef1a_Cas9-VP16 MammoBlock
  • ID: BBa_K1179002
  • Type: Generator
  • Length: 5141
  • Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with Cas9 binding sites upstream.

    Name: Hef1a_eYFP-4x451 MammoBlock
  • ID: BBa_K1179072
  • Type: Generator
  • Length: 2085
  • Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.