Team:Heidelberg/Parts

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Revision as of 02:26, 5 October 2013 by Hetitus (Talk | contribs)

Our Parts. Fascinating stuff out of the world of non-ribosomal peptides.

Team Parts

Our favorite part is BBa_K1152007: Helper construct for NRP-Indigoidine-tagging.
  • Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
  • Compatibility with custom non-ribosomal peptide (NRP) synthesis.
  • Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.

    We propose BBa_K1152013 as Best new BioBrick Part, Natural, because:
  • Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
  • Compatibility with custom non-ribosomal peptide (NRP) synthesis.
  • Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
  • Highly efficient (i.e. 0 % false-positive rate) cloning due to negative selection with ccdB.
  • Compatibility with custom non-ribosomal peptide (NRP) synthesis.
  • Optimized fusion of the NRP to Indigoidine that serves as a tag that eases detection of positive clones that synthesize the desired construct.
  • Thanks to