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19/08/13
From 2013.igem.org
(Difference between revisions)
(→Running both denatured and sonicated samples on gel) |
(→Running both denatured and sonicated samples on gel) |
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[[File:igem_denatured_son_190813.jpg]] | [[File:igem_denatured_son_190813.jpg]] | ||
*The last 6 wells after ladder are the denatured DNA samples. | *The last 6 wells after ladder are the denatured DNA samples. | ||
| - | **We were expecting to see fragmentation for the | + | **We were expecting to see fragmentation for the denatured samples. |
**There is no significant difference in between the sonicated samples and the sonicated and denatured ones. | **There is no significant difference in between the sonicated samples and the sonicated and denatured ones. | ||
**There is a slight decrease in bands depending on the sonication time interval. | **There is a slight decrease in bands depending on the sonication time interval. | ||
*Suggestions are to use ultra-sonification to view the effects with sonication focused just on sample, whereas the previous one was sonication water bath. | *Suggestions are to use ultra-sonification to view the effects with sonication focused just on sample, whereas the previous one was sonication water bath. | ||
Revision as of 14:02, 19 August 2013
Denaturing sonicated DNA
- Denaturing DNA by heat shock for 10mins in a 95C heat block
- Adding 5ul of OG dye
Running both denatured and sonicated samples on gel
- For just the sonication samples 1-6, 5ul of OG and 10ul of sterile water was added to 5ul of DNA.
- 5ul of 1kb ladder was used for 3 wells each.
- The last 6 wells after ladder are the denatured DNA samples.
- We were expecting to see fragmentation for the denatured samples.
- There is no significant difference in between the sonicated samples and the sonicated and denatured ones.
- There is a slight decrease in bands depending on the sonication time interval.
- Suggestions are to use ultra-sonification to view the effects with sonication focused just on sample, whereas the previous one was sonication water bath.
