Team:Arizona State/Notebook

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{{:Team:Arizona_State/Header}}
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This is a template page. READ THESE INSTRUCTIONS.
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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<script>
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document.title = "Notebook";
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$(document).ready(function() {
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    $("#accordion").accordion("option", "animated", 'slide');
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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</body>
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!align="center"|[[Team:Arizona_State|Home]]
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<h2>May: Week 1</h2>
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!align="center"|[[Team:Arizona_State/Team|Team]]
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<ul>
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Arizona_State Official Team Profile]
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<li>First ASU iGEM 2013 with new team members</li>
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!align="center"|[[Team:Arizona_State/Project|Project]]
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<li>Introduction to the competition, registration logistics, summer schedules</li>
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!align="center"|[[Team:Arizona_State/Parts|Parts Submitted to the Registry]]
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<li>Idea Brainstorming</li>
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!align="center"|[[Team:Arizona_State/Modeling|Modeling]]
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<li>Review of biobrick cloning, Golden Gate assembly, safety training</li>
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!align="center"|[[Team:Arizona_State/Notebook|Notebook]]
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</ul>
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!align="center"|[[Team:Arizona_State/Safety|Safety]]
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!align="center"|[[Team:Arizona_State/Attributions|Attributions]]
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|}
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<h2>Week 2</h2>
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<ul>
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<li>Idea Brainstorming--narrowed down to two project ideas</li>
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</ul>
 +
<h4>Safety Training:</h4>
 +
<ul>
 +
<li>Biosafety and Bloodborne Pathogens</li>
 +
<li>Lab Safety</li>
 +
<li>Fire Safety</li>
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<li>Recombinant DNA Safety</li>
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<li>Hazardous Waste Management</li>
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</ul>
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<h2>June: Week 3</h2>
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<ul>
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<li>Started making amp plates in preparation for experimentation</li>
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</ul>
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
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<h2>Week 4</h2>
 +
<ul>
 +
<li>Started growing up colonies of E. coli K12 MG1655, BL21 (DE3),and N10 B strains along with glycerol stocks</li>
 +
<li>Performed a restriction, ligation and transformation of the three plasmids and grew them up on a plate</li>
 +
<li>No colony growth of any of the plasmids</li>
 +
</ul>
 +
 
 +
<h2>Week 5</h2>
 +
<ul>
 +
<li>Performed a PCR amplification of the cadA promoter along with a gel screen that found the band at the appropriate band length</li>
 +
<li>Transformed and plated both J61100 and E1010 with no growth on either plates</li>
 +
<li>Gel screen of yebF without the stop codon never showed up on the gel. Ran transformation again.</li>
 +
<li>Restricted and ligated cadA and BN promoters</li>
 +
</ul>
 +
 
 +
<h2>Week 6</h2>
 +
<ul>
 +
<li>The gel screen of yebF without the stop codon was finally successful</li>
 +
<li>Transformation of INPNC-MCS and RFP control onto N10B cells was successful</li>
 +
<li>No growth from transformation of E0020, E0030, and E0040</li>
 +
<li>Created liquid cultures of E0240 and pCadA in LB Chloro.</li>
 +
</ul>
 +
 
 +
<h2>July: Week 7</h2>
 +
<ul>
 +
<li>Performed restriction digest of pCadA, pBN, and YebF</li>
 +
<li>Performed seven different ligations</li>
 +
<li>Miniprepped and nanodropped E0240 and pCadA.</li>
 +
<li>Miniprepped YebF+GMCSF and PelB leader sequence.</li>
 +
<li>Flow cytometry indicated that there was no GFP</li>
 +
<li>Transformed J23100 yet no plate growth</li>
 +
</ul>
 +
 
 +
<h2>Week 8</h2>
 +
<ul>
 +
<li>Ordered Antigen primers</li>
 +
<li>Ligation and transformation of PelB+GMCSF+pSB1C3/4K5</li>
 +
<li>Miniprep showed no successful ligation and transformation</li>
 +
</ul>
 +
 
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<h2>Week 9</h2>
 +
<ul>
 +
<li>PCR of MelanA and Flu M1 worked via confirmation from gel screen</li>
 +
<li>Nissle would not grow</li>
 +
<li>Transformed I13522 into N10B on LB Amp</li>
 +
</ul>
 +
 
 +
<h2>August: Week 11</h2>
 +
<ul>
 +
<li>Grew up six cultures of ligations</li>
 +
<li>Miniprepped the PSB1c3 backbone and grew up additional backbone in cultures</li>
 +
<li>Transformed the GFP and GMCSF in the backbone</li>
 +
<li>Ran LLO pcr on gel and all had correct band lengths</li>
 +
</ul>
 +
 
 +
<h2>September: Week 15</h2>
 +
<ul>
 +
<li>Made ligations of FLU+1c3, MelA+1c3, Flu+J04..., MelA+J04...</li>
 +
<li>Made liquid cultures of non-red colonies from FLU+1c3, MelA+1c3, Flu+J04..., MelA+J04 transformation</li>
 +
<li>Biobricks made and sent: LLO with promoter RBS, YEb-GMCSF, GMCSF, FLU1, and MelanA</li>
 +
</ul>
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</html>

Latest revision as of 23:31, 27 September 2013

May: Week 1

  • First ASU iGEM 2013 with new team members
  • Introduction to the competition, registration logistics, summer schedules
  • Idea Brainstorming
  • Review of biobrick cloning, Golden Gate assembly, safety training

Week 2

  • Idea Brainstorming--narrowed down to two project ideas

Safety Training:

  • Biosafety and Bloodborne Pathogens
  • Lab Safety
  • Fire Safety
  • Recombinant DNA Safety
  • Hazardous Waste Management

June: Week 3

  • Started making amp plates in preparation for experimentation

Week 4

  • Started growing up colonies of E. coli K12 MG1655, BL21 (DE3),and N10 B strains along with glycerol stocks
  • Performed a restriction, ligation and transformation of the three plasmids and grew them up on a plate
  • No colony growth of any of the plasmids

Week 5

  • Performed a PCR amplification of the cadA promoter along with a gel screen that found the band at the appropriate band length
  • Transformed and plated both J61100 and E1010 with no growth on either plates
  • Gel screen of yebF without the stop codon never showed up on the gel. Ran transformation again.
  • Restricted and ligated cadA and BN promoters

Week 6

  • The gel screen of yebF without the stop codon was finally successful
  • Transformation of INPNC-MCS and RFP control onto N10B cells was successful
  • No growth from transformation of E0020, E0030, and E0040
  • Created liquid cultures of E0240 and pCadA in LB Chloro.

July: Week 7

  • Performed restriction digest of pCadA, pBN, and YebF
  • Performed seven different ligations
  • Miniprepped and nanodropped E0240 and pCadA.
  • Miniprepped YebF+GMCSF and PelB leader sequence.
  • Flow cytometry indicated that there was no GFP
  • Transformed J23100 yet no plate growth

Week 8

  • Ordered Antigen primers
  • Ligation and transformation of PelB+GMCSF+pSB1C3/4K5
  • Miniprep showed no successful ligation and transformation

Week 9

  • PCR of MelanA and Flu M1 worked via confirmation from gel screen
  • Nissle would not grow
  • Transformed I13522 into N10B on LB Amp

August: Week 11

  • Grew up six cultures of ligations
  • Miniprepped the PSB1c3 backbone and grew up additional backbone in cultures
  • Transformed the GFP and GMCSF in the backbone
  • Ran LLO pcr on gel and all had correct band lengths

September: Week 15

  • Made ligations of FLU+1c3, MelA+1c3, Flu+J04..., MelA+J04...
  • Made liquid cultures of non-red colonies from FLU+1c3, MelA+1c3, Flu+J04..., MelA+J04 transformation
  • Biobricks made and sent: LLO with promoter RBS, YEb-GMCSF, GMCSF, FLU1, and MelanA

Retrieved from "http://2013.igem.org/Team:Arizona_State/Notebook"