Team:BYU Provo/Notebook

From 2013.igem.org

(Difference between revisions)
Line 151: Line 151:
: Add description of Cholera-Detection Team
: Add description of Cholera-Detection Team
-
Our focus is two-fold: 1) develop a system in ''E.coli'' to detect the presence ''V. cholerae'', and 2) integrate ''E.coli'' bacteriophage lambda into a biofilm-degrading machine that is moderated by our detection system. We continued work that last year's iGEM team began on cloning cholera's quorum-sensing circuit into a "superplasmid," but upon discovering experimentally that bacteriophage lambda can naturally sense cholera, our later research efforts center synthetically improving this pathway.  
+
Our focus is two-fold: 1) develop a system in ''E.coli'' to detect the presence ''V. cholerae'', and 2) integrate ''E.coli'' bacteriophage lambda into a biofilm-degrading machine that is moderated by our detection system. Upon discovering experimentally that bacteriophage lambda can naturally sense cholera, our research efforts have centered on synthetically improving this pathway.  
</font>
</font>

Revision as of 20:56, 27 September 2013



Phage Team

BYULPProjectPic.JPG

Large Phage Team

Bryan Merrill and Keltzie Westra
The focus of the Large Phage team is to make a cross section of phage sizes through the mutagenesis of T4 bacteriophage. Our main area of research was in making T4's already large capsid even larger, while maintaining phenotypic stability. This was done by taking the isolated large phage and observing there later generations to ensure that their phenotype is inherited.



March-April
May-June
July-August
September-October


add picture of

Small Phage Team

Xiuqi (Jade) Li and LJ Perry
We are creating a library of different size T7 bacteriophage capsids. This is accomplished by mutating the phage with a base analog and then isolating the smaller and bigger T7 phages. We are also establishing the relationship between the capsid size and the plaque size of certain phages.



March-April
May-June
July-August
September-October


Cesiumchloridegradient.jpg

Phage Purification Team

Amber Brown, Arick Christopher, and Darren Lasko
As members of the phage purification group, we focus on the purification of bacteriophages and the preparation of ghost capsids. We have worked with several different methods of preparation and can manipulate the procedures to accommodate the type of phage and the capsid size of the mutants.



March-April
May-June
July-August
September-October



Cholera Team

add picture of

Cholera-Detection Team

Clarice Harrison, Kendall Kiser, and Kelton Peck
Add description of Cholera-Detection Team

Our focus is two-fold: 1) develop a system in E.coli to detect the presence V. cholerae, and 2) integrate E.coli bacteriophage lambda into a biofilm-degrading machine that is moderated by our detection system. Upon discovering experimentally that bacteriophage lambda can naturally sense cholera, our research efforts have centered on synthetically improving this pathway.



March-April
May-June
July-August
September-October


BYU2013-CholeraGroup1.jpg

Cholera-Enzyme Team

Nathan Sabin and Michael Schellhous
On the Cholera-Enzyme team our focus is researching enzymes that will degrade the V. cholerae biofilm. Our goal is to find enzymes that have potential to degrade the biofilm, isolate them, clone them into E. coli, and design an assay to test their biofilm-degrading ability.



March-April
May-June
July-August
September