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Team:BYU Provo/Notebook/Cholera - Enzyme/July-August/Period3/Dailylog
From 2013.igem.org
| Cholera - Enzymes Notebook: May 1 - May 14 Daily Log
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8/5/13 We got a frozen culture of V. cholerae from Dr. Robison’s lab. Cells are in 20% glycerol. We are waiting for the DspB colony PCR to finish so that we can grow up both the DspB and Savinase at the same time to sequence them and get them ready for protein purification.
8/7/13 The DspB colony PCR didn’t work. We re-ran the PCR yesterday, so we will run the product on gel today, clean up the PCR and sequence it. We also prepped the Savinase for sequencing today since the DspB didn’t work and we don’t want to wait any longer. We also streaked a new plate of cholera with the culture that we received from Dr. Robison’s lab. New plate was placed in the 37° incubator.
8/9/13 Phusion PCR of Savinase and DspB. We ran two samples of each with a control:
8/12/13 Insert Text
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