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Contents 1 July 1.1 7/17/13 1.2 7/19/13 1.3 7/22/13 1.4 7/24/13 1.5 7/26/13 1.6 7/29/13 1.7 7/31/13

July

7/17/13

Today I did another round of mutagenesis using the lysis inhibition technique. I did two flasks. The first flask had 20 ug/mL of adenine which was added with the bacteria and 25 mL of LB broth; the second flask had 40 ug/mL of adenine added with the bacteria and 25 mL of LB broth. Both flasks incubated at 37C on the shaker for 3.5 hrs until slightly turbid. I then added 200 ug/mL of bromodeoxyuridine, 20 ug/mL of uracil, and .5 mL of phage to flask one; I added 400 ug/mL of bromodeoxyuridine, 40 ug/mL of uracil, and .5 mL of phage to flask two. They incubated in the same location for 3 hours.

The purification group also centrifuged (in CsCl gradient) the T4 stock and the T4 LB mutagenesis / lysis inhibition sample. Hopefully they will return it to us separated into individual fractions.

7/19/13

7/22/13

7/24/13

7/26/13

7/29/13

7/31/13