Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Dailylog

From 2013.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 6: Line 6:
| colspan="3" | <font color="#333399" size="5" font face="Calibri">  
| colspan="3" | <font color="#333399" size="5" font face="Calibri">  
-
: '''Phage Purification September - October Notebook: September 1 - September 14 Daily Log'''</font>
+
: '''Phage Purification September - October Notebook: October 14 - October 20 Daily Log'''</font>
<br>
<br>
Line 12: Line 12:
|- valign="top"
|- valign="top"
-
| style="width: 18%; background-color: transparent;"|
+
| style="width: 20%; background-color: transparent;"|
<font color="#333399" size="3" font face="Calibri">
<font color="#333399" size="3" font face="Calibri">
-
: [[Team:BYU_Provo/Phage_Purification|Overview]]
+
<font size = "4">
 +
 
 +
: <u> '''Phage Purification''' </u> </font>
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]
Line 28: Line 30:
</font>
</font>
-
| style="width: 82%; background-color: transparent;"|
+
 
 +
 
 +
| style="width: 80%; background-color: transparent;"|
<font face="Calibri" size="3">
<font face="Calibri" size="3">
<br>
<br>
-
<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period11/Dailylog"><< Previous</a>
+
<html> <a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Winterexp/Period11/Dailylog"><< Previous</a>
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period3/Dailylog" style="display:block;float:right;">Next >></a>
<a href="https://2013.igem.org/Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period3/Dailylog" style="display:block;float:right;">Next >></a>
Line 40: Line 44:
<br>
<br>
-
<font size="4"> '''9/4/13''' </font>
+
<font size="4"> '''10/16/2013''' </font>
-
- Went over the results of our T4 and T7 purification with the Large Phage and small phage group.
+
Took TEMs of mutated T7 phage for the small phage group. Found 3 small mutants and 1 large. We will repeat this experiment to get more measurements on capsid size.
-
- Updated wiki and discussed plans to have everything completed by the deadlines.
+
 +
:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/10.16T7EM| 10.16 T7 EM]]
AB DL AC
AB DL AC
<br>
<br>
-
 
-
<font size="4"> '''9/6/13''' </font>
 
-
 
-
Continued updating wiki.
 
-
 
-
AB DL AC
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''9/7/13''' </font>
 
-
 
-
We ran a T7 purification today to see if the regular WT T7 bands at a new location with our new CsCl stock.
 
-
:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient| 9.7 CsCl Gradient]]
 
-
 
-
AB DL AC
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''9/9/13''' </font>
 
-
 
-
We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.
 
-
 
-
:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
 
-
 
-
AB DL AC
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''9/11/13''' </font>
 
-
 
-
- We went ahead and ran another phage purification procedure with the new phage suspension buffer created in [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
 
-
: -[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]]
 
-
 
-
AB DL AC
 
-
 
-
<br>
 
-
 
-
<font size="4"> '''9/13/13''' </font>
 
-
 
-
- We discussed the results from [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]] and determined a new gradient to be run.  Our plan is to run three gradients next class, one wild type and two mutant gradients.
 
-
 
-
: Wild type gradient: 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8
 
-
 
-
: Mutant gradient 1: 1.2, 1.2960, 1.2966, 1.2975, 1.2981, 1.2989, 1.2997, 1.3, 1.4, 1.6
 
-
 
-
: Mutant gradient 2: 1.3, 1.3003, 1.3011, 1.3019, 1.3025, 1.3034, 1.3040, 1.4, 1.5, 1.6
 
-
 
-
- We also worked on our team profile and updated the notebook on the wiki.
 
-
 
-
AB DL AC
 
</font>
</font>

Latest revision as of 21:02, 21 October 2013


Phage Purification September - October Notebook: October 14 - October 20 Daily Log



Phage Purification
March-April
May-June
July-August
September-October



<< Previous Next >>


10/16/2013

Took TEMs of mutated T7 phage for the small phage group. Found 3 small mutants and 1 large. We will repeat this experiment to get more measurements on capsid size.

- 10.16 T7 EM

AB DL AC


<< Previous Next >>