<p style="text-align:justify">Since the Daci-Cluster genes of <i>D. acidovorans</i>, which are considered to be crucial for the production of delftibactin consist of more than 58 kb, we had to think of a method capable of trasferring this great amount of genetic information to our host organism <i>E. coli</i>. Being confronted with this task, we considered the usage of so called <i>"Bacterial Artificial Chromosomes (BACs)"</i> or <i>"Cosmids</i>. But finally, we decided to face the challege by splitting up the crucial genes of the cluster into convetional plasmids. One containing the gigantic gene "DelH", which comprises 18 kbp and another containing all residual parts, herein referred to as "DelRest". Additionally, we planed to integrate <i>D. acidovorans</i>'s methyl-malonyl-CoA-pathway, which is a crucial co-factor for one of the PKS domains and hence, for succesful delftibactin production. Because we were unfortunately not able to genomically integrate the pathway after several attempts, we decided to create a third plasmid not only containing the pathway, but as well a permeability device (BBa_...) for enabeling our bacteria to secrete the desired product. These three plasmids were constructed in different subgroups and therefore have separated labjournals. Follow the links below to read more!