Team:Heidelberg/Delftibactin/DelRest

From 2013.igem.org

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                                   <h1>Week 15</h1>
                                   <h1>Week 15</h1>
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                                   <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">
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Single read sequencing carried out by GATC and consequent alignment of the sequencing results obtained from <i>D. acidovorans</i> DSM-39 and the reference Sequence of <i>D. acidovorans SPH-1</i> (6.8 Mbp, NCBI Genome) revealed significant differences between the two strains of <i>D. acidovorans</i>. As result of this sequence analysis, the strain D. acidovorans SPH-1 was ordered from the DSMZ to have the suitable template for the primers we designed so far. Furthermore this should avoid issues which might occure in the Gibson Assembly in case mismachtes in the primers to the sequence of DSM-39 are present. Consequently all amplifications carried out before week 15 have to be repeated, conditions of the establihsed PCRs will be maintained, conditions for the missing fragments will be further optimized.
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Afterwards all fragments will be validated by restriction digests and single read seqencing.
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Revision as of 13:35, 2 October 2013

Del Rest. Creating a 32 kbp plasmid.

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