Team:Heidelberg/Delftibactin/DelRest

From 2013.igem.org

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                                   <h1>Week 16</h1>
                                   <h1>Week 16</h1>
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Last week we successfully amplified all the desired genes from the Delftibactin cluster of <i>D. Acidovorans </i>. Furthermore we were able to validate our amplicons with restriction digest ans sequencing. Therefore this weeks goal will be the cloning of our final pFSN plasmid using Gibson Assembly. Afterwards DH10B cells will be transformed with our plasmid by electroporation. Screening PCRs, test restriction digests and sequencings will be conducted to validate the final construct. Afterwards plasmids will be preped for further use and glycerol stocks of DH10B-pFSN will be made.
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Last week we successfully amplified all the desired genes from the Delftibactin cluster of <i>D. Acidovorans </i> SPH-1.
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The recently obtained strain indeed, improved our PCRs significantly. Furthermore we were able to validate our amplicons with restriction digests. Therefore this weeks goal will be the cloning of our final pFSN plasmid using Gibson Assembly. Afterwards DH10B cells will be transformed with our plasmid by electroporation. Screening PCRs, test restriction digests and sequencings will be conducted to validate the final construct. Afterwards plasmids will be preped for further use and glycerol stocks of DH10B-pFSN will prepared for further storage.
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Revision as of 20:08, 2 October 2013

Del Rest. Creating a 32 kbp plasmid.

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