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Team:Heidelberg/Safety
From 2013.igem.org
Project
Notebook
Human Practice
Safety. We take good care of you!
The uttermost anxiety of those who do not work with genetically modified organisms is concerning safety. We face this critical issue with a tripartite approach. Safety precautions concerning our project can be broken down to the following three topics: Firstly, safety considerations about the wet-lab project, secondly, the general lab safety and thirdly, software safety. In all of the three strands, we deliberated useful approaches and searched communication with safety advisors. Herewith, we wanted to get additional input which we could use for building up our opinion on which parts of the project could be critical or which measures would have to be taken.
Del H
The largest part of the pathway was amplified in subdivided fragments and subsequently assembled to a 22 kb plasmid, which was later co-transformed with the other plasmids.
Del Rest
"DelRest" consists of the genes DelA, B, C, D, E, F, G, L, O ad P, which encode NRPS- as well as PKS (polyketide synthethase) modules. We divided these genes on the cluster into certain fragments, which were amplified with Gibson-overlaps for subsequent assembly.
Methylmalonyl-CoA Pathway
After our attempts on trying to genomically integrate this pathway into E. coli didn't work out well, we created a third plasmid, on which we wanted to integrate it. Additionally we therafter built in a permeability device from the parts-registry for delftibactin secretion into the medium.
