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| | {{:Team:Heidelberg/Templates/Navigation}} | | {{:Team:Heidelberg/Templates/Navigation}} |
| | <html> | | <html> |
| | + | <style type="text/css"> |
| | + | .carousel-inner{ |
| | + | height:540px; |
| | + | } |
| | + | </style> |
| | + | <div class="row"> |
| | + | <div class="col-md-8 col-md-offset-2"> |
| | <!-- Carousel | | <!-- Carousel |
| | ================================================== --> | | ================================================== --> |
| | | | |
| - | <div style="margin-bottom:2%;"> | + | <div> |
| - | <div id="myCarousel" class="carousel slide"> | + | <div id="myCarousel" class="carousel slide" style="margin-top:15%"> |
| | <!-- Indicators --> | | <!-- Indicators --> |
| - | <ol class="carousel-indicators"> | + | <ol class="carousel-indicators" style="position:absolute; top:80%"> |
| | <li data-target="#myCarousel" data-slide-to="0" class="active"></li> | | <li data-target="#myCarousel" data-slide-to="0" class="active"></li> |
| | <li data-target="#myCarousel" data-slide-to="1"></li> | | <li data-target="#myCarousel" data-slide-to="1"></li> |
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| | <div class="carousel-inner"> | | <div class="carousel-inner"> |
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| - | <div class="item active may first"> | + | <div class="item active"> |
| - | <img src="Heidelberg_3.JHD.jpg"> | + | <img src="https://static.igem.org/mediawiki/2013/6/66/Heidelberg_IMAG0449.png" alt="" /> |
| - | <div class="container">
| + | |
| - | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0" id="week1"> | + | <div class="carousel-caption"> |
| | <h1>Week 1</h1> | | <h1>Week 1</h1> |
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| | </div> | | </div> |
| - | </div>
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| - | </div>
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| - | <div class="item may last">
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| - | <div class="container">
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| - | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0">
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| - | <h1>Week 3</h1>
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| - | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">We were able to dissolve gold-containing parts of an old CPU and established a protocol for recovery of gold as soluble gold salts from electronic waste. Moreover, we were able to show specific precipitation of solid gold by <i>D. acidovorans</i> from this "dissolved electronic waste".
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| - | </p>
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| - | </div>
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| - | </div>
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| - | </div>
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| | | | |
| - | <div class="item august first">
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| - | <div class="container">
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| - | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0">
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| - | <h1>Week 15</h1>
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| - | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Using supernatants from the new <i>Delftia acidovorans</i> strain SPH-1, we showed precipitation of gold chloride solution to gold nanoparticles. Furthermore, we melted the purple-black nanoparticles to shiny solid gold. </p>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | <div class="item august last">
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| - | <img src="data:image/png;base64,"/>
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| - | <div class="container">
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| - | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0">
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| - | <h1>Week 19</h1>
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| - | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">We optimized growth conditions of D. acidovorans and evaluated endogenous background precipitation of metall ions in the possible target E. coli strains E. coli DH10ß and BL21 DE3 following incubation over night on LB and ACM plates. <i>Delftia acidovorans</i> did not exceed <i>E. coli</i>, most probably due to insufficient cultivation time. When grown on LB plates, neither <i>D. acidovorans</i> nor <i>E. coli</i> showed any reactivity. </p>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | <div class="item september first">
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| - | <img src="data:image/png;base64,"/>
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| - | <div class="container">
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| - | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0">
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| - | <h1>Week 20</h1>
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| - | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">We continued optimized growth conditions of <i>D. acidovorans</i> and testing various <i>E. coli</i> strains for their endogenous capability to precipitate gold in order to identify the strain with the least background to be used as target strain after 2 and 3 days as well as grown at 30°C and room temperature. A cultivation at 30°C for 3 days was identified as optimal. We also started to establish purification of Delftibactin using HP20 resins and successfully verified presence of Delftibactin in the supernatant of <i>D. acidovorans</i> SHP-1. Additionally, we proved precipitation of gold by the purified Delftibactin and detected it by Micro-TOF File:20130911Malditof.pdf. Moreover, we triple-electroporated the final DelRest construct, the final MMCoA plasmid and the first promissing DelH clone into <i>E. coli</i> DH10ß. The Micro-TOF has to be repeated again next week. </p>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | <div class="item september">
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| - | <img src="data:image/png;base64,"/>
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| - | <div class="container">
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| - | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0">
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| - | <h1>Week 21</h1>
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| - | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">We repeated last week's Micro-TOF of the first promossing triple-clone, which did not show detectable expression of Delftibactin. </p>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | <div class="item september">
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| - | <img src="data:image/png;base64,"/>
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| - | <div class="container">
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| - | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0">
| |
| - | <h1>Week 22</h1>
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| - | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">Possible E. coli target strains BL21 DE, DH10ß and NEB Turbo were analyzed for their background expression and indcibility. E. coli BL21 DE was identified as best of these three. It was electroporated with DelRest and pIK8.6 and of these, electrocompetent cells were prepared. </p>
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| - | </div>
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| - | </div>
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| - | </div>
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| - | <div class="item september last">
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| - | <img src="data:image/png;base64,"/>
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| - | <div class="container">
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| - | <div class="carousel-caption scrollContent2" data-spy="scroll" data-target="#navbarExample" data-offset="0">
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| - | <h1>Week 23</h1>
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| - | <p style="font-size:10pt; text-align:justify; position:relative; margin-left:6%;">E. coli BL21 DE + DelRest + pIK8.6 were elctroporated with the DelH clone C5, which harbors a amino acid substitution at the beginning of DelH. Its capability to precipitate gold from solution was analyzed on ACM plates following induction by IPTG. Due to a contamination, the results were inconclusive. The production of Delftibactin was accessed by Micro-TOF. </p>
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| - | <p><a class="btn btn-large btn-primary" href="#">Browse gallery</a></p>
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| - | </div>
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| | </div> | | </div> |
| - | </div>
| + | |
| | <a class="left carousel-control" href="#myCarousel" data-slide="prev"><span class="glyphicon glyphicon-chevron-left"></span></a> | | <a class="left carousel-control" href="#myCarousel" data-slide="prev"><span class="glyphicon glyphicon-chevron-left"></span></a> |
| | <a class="right carousel-control" href="#myCarousel" data-slide="next"><span class="glyphicon glyphicon-chevron-right"></span></a> | | <a class="right carousel-control" href="#myCarousel" data-slide="next"><span class="glyphicon glyphicon-chevron-right"></span></a> |
| | </div> | | </div> |
| | </div><!-- /.carousel --> | | </div><!-- /.carousel --> |
| - | </div>
| + | </div> |
| | + | </div> |
| | + | </div> |
| | + | <script rel="" type="text/javascript"> |
| | + | $(function(){ |
| | + | $('.carousel').carousel() |
| | + | }); |
| | + | </script> |
| | </html> | | </html> |
| | {{:Team:Heidelberg/Templates/Footer-Nav}} | | {{:Team:Heidelberg/Templates/Footer-Nav}} |
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