Team:KU Leuven/Project/HoneydewSystem

From 2013.igem.org

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       <p align="justify">We have modeled the production system of methyl salicylate by using the transcription, translation and protein degradation rate in order to calculate the mRNA and protein flux. We also brought the kinetics of methyl salicylatesynthesis into account.</p>
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       <p align="justify">We have modeled the production system of methylsalicylate by using the transcription, translation and protein degradation rate in order to calculate the mRNA and protein flux. We also brought the kinetics of methylsalicylatesynthesis into account.</p>
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Revision as of 17:54, 2 October 2013

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon

Short text about glucose model

E-β-Farnesene

EBF serves as the most universal aphid alarm pheromone. It is released from the cornicles of the aphids to warn others against upcoming danger, such as the natural predators of aphids. Because of the fact that EBF is highly susceptible to oxidation, we want to make our BanAphids produce EBF regularly.

MethylSalicylate - Wetlab

Methylsalicylate is a pheromone released by plants when they are attacked by aphids. It activates plant defence systems, as well as attract predators of the aphids, such as the ladybug or the green lacewing. In the lab we have focused on increasing the production of methylsalicylate of an existing brick, by increasing the production of its precursor, chorismate.

MethylSalicylate - Modelling

We have modeled the production system of methylsalicylate by using the transcription, translation and protein degradation rate in order to calculate the mRNA and protein flux. We also brought the kinetics of methylsalicylatesynthesis into account.

qPCR

We did a qPCR to check whether our genes of interest are properly transcribed. Also, the amount of mRNA we can measure with qPCR could serve as input data for our methylsalicylate model.