Team:MIT/Project
From 2013.igem.org
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<h3>(7) Testing our Protein Signal</h3> | <h3>(7) Testing our Protein Signal</h3> | ||
We demonstrated retained functionality of our protein signal after fusion with Acyl-TyA (Acyl-TyA-rtTA3). In addition, we tested our reporter construct (TRE-tight_mkate), which allowed us to assay for the function of our protein signal.<br><br> | We demonstrated retained functionality of our protein signal after fusion with Acyl-TyA (Acyl-TyA-rtTA3). In addition, we tested our reporter construct (TRE-tight_mkate), which allowed us to assay for the function of our protein signal.<br><br> | ||
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<h3>(10) Application - Endogenous Gene Activation</h3> | <h3>(10) Application - Endogenous Gene Activation</h3> | ||
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We tested our Cas9-VP16 fusion protein by transfecting it into HEK 293 cells along with a guide RNA which will target the Cas9-VP16 to our reporter construct and activate the expression of EYFP. We showed that our Cas9-VP16 fusion is indeed functional. <br><br> | We tested our Cas9-VP16 fusion protein by transfecting it into HEK 293 cells along with a guide RNA which will target the Cas9-VP16 to our reporter construct and activate the expression of EYFP. We showed that our Cas9-VP16 fusion is indeed functional. <br><br> | ||
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Revision as of 03:35, 26 October 2013