Team:MIT/ResultsBiobricks

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Revision as of 19:39, 28 October 2013 by Klathem (Talk | contribs)

iGEM 2012

Overview

  • Project Overview

miRNA Signal

  • Overview
  • siRNA Characterization
  • Exosome Isolation and Co-Culturing
  • Cell-Cell Co-Culturing

Protein Signals

  • Overview
  • GFP
  • rtTA3
  • Cre
  • L7Ae
  • Cas9-VP16

Novel DNA Sensor: Cas9 Split Venus Fusion

  • Overview
  • Leucine Zipper Fusion
  • DNA Sensing

Our BioBricks

  • Favorites
  • All BioBricks

Attributions

  • Attributions

Biobricks


Favorite Parts:

Name: CMV_Acyl-TyA-eGFP MammoBlock
  • ID: BBa_K1179071
  • Type: Generator
  • Length: 2834
  • Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence.

    Name: Hef1a_Cas9-VP16 MammoBlock
  • ID: BBa_K1179002
  • Type: Generator
  • Length: 5141
  • Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with Cas9 binding sites upstream.

    Name: Hef1a_eYFP-4x451 MammoBlock
  • ID: BBa_K1179072
  • Type: Generator
  • Length: 2085
  • Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.

    Acyl-TyA BBa_K1179000
    Acyl-TyA-eGFP BBa_K1179001
    Hef1a_Cas9-VP16 BBa_K1179002
    Acyl-TyA-mKate-His BBa_K1179003
    Acyl-TyABBa_K1179000
    Acyl-TyA-eGFPBBa_K1179001
    Hef1a_Cas9-VP16BBa_K1179002
    Acyl-TyA-mKate-HisBBa_K1179003
    Acyl-TyA-rtTABBa_K1179004
    Acyl-TyA-rtTA-HisBBa_K1179005
    Acyl-TyA-TevBBa_K1179006
    Acyl-TyA-TEV-rtTA-HisBBa_K1179007
    Cas9 D10A & H840A mutation-linkerBBa_K1179008
    Cas9-Linker-VP16BBa_K1179009
    eBFPBBa_K1179010
    eYFPBBa_K1179012
    eYFP-PESTBBa_K1179013
    VP16-GAL4BBa_K1179014
    U6_gRNA (Cr9)BBa_K1179015
    L7AeBBa_K1179016
    Linker-Cas9 D10A & H840A mutationBBa_K1179017
    mKate –PESTBBa_K1179018
    mKate-HisBBa_K1179019
    rtTA-HisBBa_K1179020
    CAG_Acyl-TyA-mKate-HisBBa_K1179021
    CAG_Acyl-TyA-rtTABBa_K1179022
    CAG_Acyl-TyA-rtTA-HisBBa_K1179023
    CAG_eYFP-PESTBBa_K1179024
    CAG_L7AeBBa_K1179025
    CAG_mKate-PESTBBa_K1179026
    CAG-tagBFPBBa_K1179027
    D10A and H840A mutant Cas9-VC155BBa_K1179028
    D10A and H840A mutant Cas9-VN155BBa_K1179029
    D10A and H840A mutant Cas9-VN173BBa_K1179030
    CMV_ L7Ae_myc-His6BBa_K1179032
    CMV_2xKMet-eGFPBBa_K1179033
    L7Ae-2a-eYFPBBa_K1179037
    TRE_Cas9-VP16BBa_K1179040
    U6_gRNA 1A ReverseBBa_K1179041
    U6_gRNA 1ABBa_K1179042
    U6_gRNA 2ABBa_K1179043
    U6_gRNA 3ABBa_K1179044
    U6_gRNA 4ABBa_K1179045
    U6_gRNA 5ABBa_K1179046
    U6_gRNA 6ABBa_K1179047
    U6_gRNA 6A ReverseBBa_K1179048
    VC155BBa_K1179049
    VC155 with Leucine zipperBBa_K1179050
    VC155-Cas9(D10A and H840A mutant)BBa_K1179051
    VN155BBa_K1179052
    VN155 with Leucine zipperBBa_K1179053
    VN155-Cas9(D10A and H840A mutant)BBa_K1179054
    VN173BBa_K1179055
    VN173 with Leucine zipperBBa_K1179056
    VN173-Cas9(D10A and H840A mutant)BBa_K1179057
    Cre entry vectorBBa_K1179058
    eYFP-4x451tsBBa_K1179060
    eYFP-4x503tsBBa_K1179061
    LacIBBa_K1179062
    LacI-4x451tsBBa_K1179063
    LacI-4x503tsBBa_K1179064
    TagBFPBBa_K1179068
    CAGBBa_K1179069
    CMV_Acyl-TyA-eGFPBBa_K1179071
    Hef1a_eYFP-4x451BBa_K1179072
    TRE-tight_eYFP-4x451BBa_K1179073
    Hef1a_eYFP-4x503BBa_K1179074
    TRE-t_eYFP-4x503BBa_K1179075

    • Retrieved from "http://2013.igem.org/Team:MIT/ResultsBiobricks"