Team:MSOE Milwaukee/Primers

From 2013.igem.org

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   <TD bgcolor = #649327 width = 200 valign = bottom height = 50><font color = "white" size = "+2" >DNA SEQUENCE/ENZYME</TD>
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   <TD bgcolor = #006331 width = 200 valign = bottom height = 50><font color = "white" size = "+2" >DNA SEQUENCE/ENZYME</TD>
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   <TD bgcolor = #649327 width = 760 valign = bottom height = 50><font color = "white" >PRIMER TO ADD THE PREFIX (FOR) 5' to 3' <br> PRIMER TO ADD THE SUFFIX (REV) 5' to 3'</TD>
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   <TD bgcolor = #006331 width = 760 valign = bottom height = 50><font color = "white" >PRIMER TO ADD THE PREFIX (FOR) 5' to 3' <br> PRIMER TO ADD THE SUFFIX (REV) 5' to 3'</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">HMG-CoA Synthase reverse</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">HMG-CoA Synthase reverse</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align= left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align= left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Kinase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Kinase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG</TD>
   
   
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate Kinase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate Kinase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Diphosphate Kinase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Diphosphate Kinase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylanase C</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylanase C</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylosidase</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylosidase</TD>
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   <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG<br>GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG</TD>
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   <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG<br>GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50><font color = "white" size = "+2">osmY with GS liner (two reverse)</TD>
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  <TD bgcolor = #9BBB59 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCCGCTTCTAGATGACTATGACAAGACTGAAG<br>GGATCCCCCGCTACCACTGCCCGAACCCTTAGTTTTCAGATCATT<br>GCTAATGATCATCGCCGGCGACGTCGGATCCCCCGCTACCACTGCCC</TD>
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Latest revision as of 21:29, 20 September 2013

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DNA SEQUENCE/ENZYME PRIMER TO ADD THE PREFIX (FOR) 5' to 3'
PRIMER TO ADD THE SUFFIX (REV) 5' to 3'
1,8-cineole synthase ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC
GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG
HMG-CoA Synthase reverse ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC
GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG
Mevalonate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG
GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG
Phosphomevalonate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC
GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC
Mevalonate Diphosphate Kinase ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG
GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC
Xylanase C ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC
GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC
Xylosidase ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG
GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG