Team:NCTU Formosa/biobricks

From 2013.igem.org

(Difference between revisions)
(Parts submitted to the Registry)
(Parts submitted to the Registry)
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===Parts submitted to the Registry===
===Parts submitted to the Registry===
<groupparts>iGEM013 NCTU_Formosa</groupparts>
<groupparts>iGEM013 NCTU_Formosa</groupparts>
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<p>[[File:Nctu_Pred.jpg]]</p>
+
<p>Ompc promtor, which can sense red light with the presence of cph8. It will be turned on in the dark ,and be turned off in the bright. For the convenient use, we add lacI and lac promotor downstream the biobrick. This part, so called red promotor, can be activated under red light, and inactive in the dark. [[File:Nctu_Pred.jpg]]</p>
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<p>[[File:Nctu_rbs2.jpg]]</p>
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<p>The sRNA, base pair with target mRNA, including the Shine-Dalgarno sequence. Thus it prevent ribosome from binding to initiate the translation. The rRBS is designed for sRNA perfect binding, and this rRBS is the RBS which can be bound only for our artificial sRNA(BBa_K1017404).[[File:Nctu_rbs2.jpg]]</p>
<p>[[File:Nctu_cph8.jpg]]</p>
<p>[[File:Nctu_cph8.jpg]]</p>
<p>[[File:Nctu_srna2.jpg]]</p>
<p>[[File:Nctu_srna2.jpg]]</p>

Revision as of 02:45, 18 October 2013

Biobricks

To strive for the best results, we made a number of experiment. Our team always hang together in spite of many losses.

Parts submitted to the Registry

<groupparts>iGEM013 NCTU_Formosa</groupparts>

Ompc promtor, which can sense red light with the presence of cph8. It will be turned on in the dark ,and be turned off in the bright. For the convenient use, we add lacI and lac promotor downstream the biobrick. This part, so called red promotor, can be activated under red light, and inactive in the dark. Nctu Pred.jpg

The sRNA, base pair with target mRNA, including the Shine-Dalgarno sequence. Thus it prevent ribosome from binding to initiate the translation. The rRBS is designed for sRNA perfect binding, and this rRBS is the RBS which can be bound only for our artificial sRNA(BBa_K1017404).Nctu rbs2.jpg

Nctu cph8.jpg

Nctu srna2.jpg

Nctu sRNA rRBS1.jpg

Nctu srna2 rRNS.jpg

Nctu plux srna1.jpg

Nctu 37rbs mGFP.jpg

Nctu 37rbs luxr.jpg

Nctu pcya ho1.jpg

Nctu lacI plac.jpg

Nctu pcons rbs2 mrfp.jpg

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