Team:NTNU-Trondheim/Notebook

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    <title>Trondheim iGEM 2013 </title>
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{{#calendar: year=2013 | title=Team:NTNU-Trondheim }}
 
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<div id="top-section3"><center><img src="https://static.igem.org/mediawiki/2013/2/21/Logo2_NTNU.png" alt="header" border="0"  usemap="#igemmap" width="1000" height="400"></center></div>
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<div class= "layout-1000" >
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<div id='cssmenu'>
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<ul>
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  <li class='active'><a href='https://2013.igem.org/Team:NTNU-Trondheim'><span>Home</span></a></li>
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  <li class='has-sub'><a href='#'><span>Project</span></a>
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      <ul>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Project'><span>Project description</span></a></li>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/novelapproach'><span>A novel approach</span></a></li>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Model'><span>Modelling</span></a></li>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Experiments_and_Results'><span>Experiments and Results</span></a></li>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Parts'><span>BioBrick Parts</span></a></li>
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        <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Acknowledgements'><span>Acknowledgements</span></a></li>
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      </ul>
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  </li>
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  <li class='has-sub'><a href='#'><span>Technical Stuff</span></a>
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      <ul>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Notebook'><span>Notebooks</span></a></li>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Protocols'><span>Protocols</span></a></li>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Primers'><span>Primers</span></a></li>
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        <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Safety'><span>Safety</span></a></li>
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      </ul>
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  </li>
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  <li class='has-sub'><a href='#'><span>Team</span></a>
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      <ul>
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        <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Team'><span>Meet the team</span></a></li>
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        <li class='last'><a href='https://igem.org/Team.cgi?id=1082'><span>Official Team Profile</span></a></li>
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      </ul>
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  </li>
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  <li class='has-sub'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Outreach'><span>Outreach</span></a>
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  </li>
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    <li class='has-sub'><a href='#'><span>Judging</span></a>
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    <ul>
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    <li><a href='https://2013.igem.org/Team:NTNU-Trondheim/Achievements'><span>Achievements</span></a></li>
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    <li class='last'><a href='https://2013.igem.org/Team:NTNU-Trondheim/Medalcriteria'><span>Medal criteria</span></a></li>
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    </ul>
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    </li>
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  <li class='last'><a href='https://2012.igem.org/Team:NTNU_Trondheim/Matchmaker'><span>Matchmaker</span></a></li>
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</ul>
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</div>
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<div> <div class ="row-end"> </div>
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</div>
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</div>
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<div id="Default">
 
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== Notebook ==
 
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{{:Team:NTNU-Trondheim/20_June_2013}}
 
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[https://2013.igem.org/Team:NTNU-Trondheim/TransformedBricks Transformed BioBricks]
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<div class="row-end"> </div>
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====Tuesday 18.06.13====
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<div class="col8" align = "center" style="background-color:#C3A7F3;>
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<p style="text-align:center; color:black; "> Notebook</p> </div>
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<div class ="row-end"> </div>
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</div>
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<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/June">June</a><br>
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Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts Promoter+RBS+RFP+term (<partinfo>BBa_I13521</partinfo>), Promoter+RBS+GFP+term (<partinfo>BBa_I13522</partinfo>), Promoter+RBS+CFP+term (<partinfo>BBa_I13600</partinfo>) and P<sub>m</sub>+RBS+mCherry+term to competent ''E.coli'' DH5&alpha; cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-)
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<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/July">July</a><br>
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<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/August">August</a><br>
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For our transformation protocol, see the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols protocol page].
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<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/September">September</a><br>
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<a href="https://2013.igem.org/Team:NTNU-Trondheim/Notebook/October">October</a><br>
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<br></br>
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====Wednesday 19.06.13====
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<a href="https://2013.igem.org/Team:NTNU-Trondheim/TransformedBricks">Transformed Biobrick</a><br>
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We transformed BioBricks consisting of fluorescent proteins. Transformed the parts YFP (<partinfo>BBa_E0030</partinfo>), CFP (<partinfo>BBa_E0020</partinfo>), RFP (<partinfo>BBa_E1010</partinfo>), GFP (<partinfo>BBa_E0040</partinfo>), BFP (<partinfo>BBa_K592100</partinfo>) and SYFP (<partinfo>BBa_K864100</partinfo>).
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We transferred mCherry from agar plate to liquid medium and incubated at 37&deg; C.
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====Thursday 20.06.13====
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We transferred the transformed cells from yesterday to liquid medium and incubated at 37&deg;C [https://2013.igem.org/Team:NTNU-Trondheim/Protocols protocol].
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We isolated the DNA from the transformed mCherry cells by using the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols miniprep protocol] and measured the concentration by NanoDrop.
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{|border="1"
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|Sample
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|Concentration (ng/&micro;l)
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|-
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|mCherry1
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|5,6
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|mCherry2
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|7,3
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|}
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Latest revision as of 22:45, 4 October 2013

Trondheim iGEM 2013

header
Mercury
Notebook

June
July
August
September
October


Transformed Biobrick


Retrieved from "http://2013.igem.org/Team:NTNU-Trondheim/Notebook"