Team:SDU-Denmark

From 2013.igem.org

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     <h2 class="onBlack">Status as of 6th of august 2013</h2>
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     <h2 class="onBlack">Status as of 27th of september 2013</h2>
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      Not much free time, but our summer has definitely been exciting so far...
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The end is coming close, and it all gets more exciting...  
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Currently we have finished two subprojects. We have cloned two genes, <i>dxs</i> from another bacteria  and <i>ispG</i>, into the <i>E. coli</i>. These genes are the limiting steps in the synthesis of IPP and are introduced into the bacteria to optimise the IPP production. Furthermore we have sequenced the <i>dxs</i> gene of <i>E. coli</i> in order to improve a preexisting biobrick.
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Is it 3:00 in the morning and the wiki is sproutling. Members of the preceding iGEM teams of SDU prepared a feast for us, so we could work without wasting time on cooking. We are in the middle of the characterization of our rubber producing bacteria, and the future looks bright. The weekend is accompanied by multiple growth experiments, GC, H-NMR, MALDI-TOF, wikiwork and rubber extraction.  
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Revision as of 01:04, 27 September 2013

Doesn't rubber come from trees?

Let your eyes (and mouse) wander to these trees to discover our ideas on how to help the environment and change the future of rubber-production. Take a look at our short project description below.

The growing demand for natural rubber causes deforestation of the rainforest or occupation of arable lands, all due to the founding of new plantations. If producing rubber by bacteria succeeds, production of natural rubber will not be limited to the regions where the rubber tree can grow but can be done even in barren lands.

Our project aims to make a common bacteria able to produce natural rubber while grown under controlled conditions. Natural rubber is composed of molecules, each consisting of the substance IPP linked together like a chain. The common bacteria that we use already possesses the ability to produce the IPP, but it lacks the enzyme to connect the IPP links together into a chain. Through this project we introduce the enzyme that the rubber tree has for connecting the links, into the bacteria. Furthermore we manipulate the bacteria into producing more of the IPP links.

Status as of 27th of september 2013

The end is coming close, and it all gets more exciting...

Is it 3:00 in the morning and the wiki is sproutling. Members of the preceding iGEM teams of SDU prepared a feast for us, so we could work without wasting time on cooking. We are in the middle of the characterization of our rubber producing bacteria, and the future looks bright. The weekend is accompanied by multiple growth experiments, GC, H-NMR, MALDI-TOF, wikiwork and rubber extraction.