Team:UCL/Labbook/Week8
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Lab Weeks
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Week 8
Bacterial Lab
Monday 22nd July - Transformation of our competent cells with plasmid YB3110 was carried out.
Tuesday 23rd July - No colony growth was observed on Ampicillin plates indicating no plasmid uptake. transformation was repeated with YB3110.
Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
---|---|---|---|
1 (Main) | Yes | Yes | 0 |
2 (Positive Control) | No | Yes | 100+ |
3 (Negative Control) | Yes | No | 0 |
Wednesday 24th July - No colony growth from main experiment indicating no plasmid uptake. Transformation was repeated.
Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
---|---|---|---|
1 (Main) | Yes | Yes | 0 |
2 (Positive Control) | No | Yes | 100+ |
3 (Negative Control) | Yes | No | 0 |
Thursday 25th July - No colony growth and hence no plasmid uptake. Transformation was repeated.
Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
---|---|---|---|
1 (Main) | Yes | Yes | 0 |
2 (Positive Control) | No | Yes | 100+ |
3 (Negative Control) | Yes | No | 0 |
Friday 26th July - Once again our cells were unsuccessful in taking up the YB3110 plasmid. We concluded that our batch of competent cells were not competent and so these will not be used in any further experiments. A new batch of competent cells are to be generated.
Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
---|---|---|---|
1 (Main) | Yes | Yes | 0 |
2 (Positive Control) | No | Yes | 100+ |
3 (Negative Control) | Yes | No | 0 |
Mammalian Lab
Monday 22nd July - MEF passage 6. Training ends.