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Team:UCL/Labbook/Week8
From 2013.igem.org
Lab Weeks
Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18
Week 8
Bacterial Lab
Monday 22nd July
Transformation of our competent cells with plasmid YB3110 was carried out.
Tuesday 23rd July
No colony growth was observed on Ampicillin plates indicating no plasmid uptake. Transformation was repeated with YB3110.
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 0 |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 0 |
Wednesday 24th July
No colony growth from main experiment indicating no plasmid uptake. Transformation was repeated.
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 0 |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 0 |
Thursday 25th July
No colony growth and hence no plasmid uptake. Transformation was repeated.
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 0 |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 0 |
Friday 26th July
Once again our cells were unsuccessful in taking up the YB3110 plasmid. We concluded that our batch of competent cells were not competent and so these will not be used in any further experiments. A new batch of competent cells are to be generated.
| Vial | Ampicillin Plate | Plasmid Insertion | Colony Count |
|---|---|---|---|
| 1 (Main) | Yes | Yes | 0 |
| 2 (Positive Control) | No | Yes | 100+ |
| 3 (Negative Control) | Yes | No | 0 |
Mammalian Lab
Monday 22nd July
MEF passage 6. Training ends.
