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Team:UNITN-Trento/Notebook/Labposts/08/43
From 2013.igem.org
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{ | { | ||
| - | + | "date":"2013-08-31", | |
| - | + | "author":"fabio", | |
| - | + | "title":" <html> the blue circuit doesn’t always work!!</html> ", | |
| - | + | "content":" <html> I repeated the experiment with light induction lots and lots of times, but unfortunately a few time cells behaved strangely: even the dark control produced amilCP... probably the circuit is not perfectly regulated!! Now I have to think about a new cloning: pedro will try out a ligation of the circuit with EFE, instead I will try to insert EFE at the end of the improved circuit without inverter. I will do a PCR of the improved part with Kapa polymesase and then inserting this sequence inside the plasmid containing EFE +terminators. Then we will have ethylene production upon illumination.</html> ", | |
| - | + | "tags":"blue_light-EFE" | |
} | } | ||
Revision as of 08:03, 25 September 2013
{ "date":"2013-08-31", "author":"fabio", "title":" the blue circuit doesn’t always work!! ", "content":" I repeated the experiment with light induction lots and lots of times, but unfortunately a few time cells behaved strangely: even the dark control produced amilCP... probably the circuit is not perfectly regulated!! Now I have to think about a new cloning: pedro will try out a ligation of the circuit with EFE, instead I will try to insert EFE at the end of the improved circuit without inverter. I will do a PCR of the improved part with Kapa polymesase and then inserting this sequence inside the plasmid containing EFE +terminators. Then we will have ethylene production upon illumination. ", "tags":"blue_light-EFE" }
