Team:UT-Tokyo/Project

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                     <li><span class="mhead">RNA Silencing</span>
                     <li><span class="mhead">RNA Silencing</span>
                     <ol class="menu">
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                        <li><a href="#rSummary">Summary</a></li>
 
                         <li><a href="#rBackground">Background</a></li>
                         <li><a href="#rBackground">Background</a></li>
                         <li><a href="#rSystems">Systems</a></li>
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<h1 id="Multicellular_Analog_Clock">Multicellular Analog Clock</h1>
<h1 id="Multicellular_Analog_Clock">Multicellular Analog Clock</h1>
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            <h2 id="mSummary">Summary</h2>
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<h2 id="mSystems">Systems</h2>
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            <p class="ini">
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<h2 id="mBackground">Background</h2>
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  <p class="ini">Knowing time has been essential to human activities. Since the ancient period, human beings have developed the way to tell the time. Even though recent technological progress enabled various types of digital clocks, analog clocks still haven’t lost its importance being intuitive to understand. In our project, we aimed to build an “analog clock” by means of making a visible excitation wave of E.coli cells. On the contrary to usual oscillators built into E.coli, we can directly see “a clock hand” i.e. an excitation wave, moving around. That is to say, we can observe and intuitively understand the spatial expression of genes without any special equipment.</p>
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            <img src="ddes.png" class="figure">
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<h3>What is the Multicellular Analog Clock?</h3>
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<p class="ini">The goal of the project is to make the Multicellular Analog Clock which has following features;</p>
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<p> There were some technological challenges to achieve this goal. How can we make an excitation wave of E.coli such that “a clock hand” moves only in one direction? We decided to imitate nerve system. Nerve system makes use of positive and negative feedback loops. Ion channels which are initially at resting state get depolarized by a stimulus. This opens the channels giving rise to higher depolarization. After certain period, the sodium channel becomes inactivated, a period called refractory period. This refractory period prevents signals to go backward, makes them travelling in one direction. By mimicking this system, we can make the clock hand move only clockwise. This approach suggests an application of the clock as a signal transfer system.</p>
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<p>1. One directional movement of position of gene expression on a multicellular scale (like the movement of clock hand).</p>
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<p>2. Easy observation; visible under natural light without any special equipment.</p>
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<p>3. Easy opreration; a familiar agar plate with <i>E. coli</i> population on it.</p>
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<img src="ddes.png" class="figure">
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<p>We designed “gear” and “clock hand”. The genetic “gear” of the clock, which generates gene expression in response to a stimulus and relays this stimulus to surrounding cells, consists of a positive feedback loop and a Multi-negative feedback system. The “clock hand” to visualize this gene expression is mCherry, a protein visibly red under natural light (Table 1). <i>E. coli</i> possessing this genetic behavior is placed on an agar plate so as to create a circle. Agar plates are relatively inexpensive and easier to use than microfluidic device, which is used to observe the spatiotemporal behavior of gene expression in previous research (Tal Danino et al., 2010 [1]). Agar plate is small and portable while microfluidic device has machines and tubes connected to it. These advantages of agar plates may contribute to the intuitive understanding of clock hand movement, and lead to future applications.</p>
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<p>One significant difference between nerve system and E.coli Clock is that former is basically a one-cell system whereas latter is a multicellular system. Therefore, we must control the cell-cell communication of E.coli to build an Analog E.coli clock. Though its importance has been emphasized, bioengineering tools for cell-cell communication control is still under development. For example, Hasty group reported[1] a synchronized quorum of genetic clock, in which synchronized oscillation was generated. However, they implemented micro fluidic device, which is not easily accessible to most of iGEM teams. Furthermore, this is merely an oscillator rather than excitation wave, as is the case for most of other “clocks”[2]. To the best of our knowledge, iGEM Chiba 2009 project[3] is the only example of clock with moving hand. However, their system is based on diffusion of substance and cannot be precisely controlled. Our project, on the other hand, constructs an excitation wave by gene expression. This system is more sophisticated than mere diffusion system and can be applied to various areas. In order to realize this novel system, we developed new bioengineering tools for cell-cell communication.</p>
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<p><b>Table 1</b></p>
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<img src="https://static.igem.org/mediawiki/2013/6/60/Tab3yamada.png" class="figure">
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Systems
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<p>The clock device also contains a “control” reporter (amilGFP) for dual-reporter assay and a UV reset device (lambda cI repressor), which are explained later.</p>
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<img src="https://static.igem.org/mediawiki/2013/0/06/1013Figure1.png"  class="figure">
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<p><b>Figure 1</b> Multicellular Analog Clock</p>
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What is the Multicellular Analog Clock?
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<img src="https://static.igem.org/mediawiki/2013/5/5d/Fig2yamada.png" class="figure">
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The goal of the project is to make the Multicellular Analog Clock which has following features;
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<p><b>Figure 2</b> The clock device. Pink box genes (luxI and luxR) form the positive feedback loop of AHL production. Blue box genes (tetR, aiiA, and sRNAs) are the suppressors used for negative feedback loops. mCherry is a red reporter protein for visualization of the excitation wave (“clock hand”). amilGFP is the yellow reporter protein expressed constitutively as an internal standard.</p>
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1. One directional movement of position of gene expression on a multicellular scale (like the movement of clock hand).
 
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2. Easy observation; visible under natural light without any special equipment.
 
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3. Easy opreration; a familiar agar plate with E. coli population on it.
 
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We designed “gear” and “clock hand”. The genetic “gear” of the clock, which generates gene expression in response to a stimulus and relays this stimulus to surrounding cells, consists of a positive feedback loop and a Multi-negative feedback system. The “clock hand” to visualize this gene expression is mCherry, a protein visibly red under natural light (Table 1). E. coli possessing this genetic behavior are placed on an agar plate so as to create a circle. Agar plates are relatively inexpensive and easier to use than microfluidic device, which is used to observe the spatiotemporal behavior of gene expression in previous research (Tal Danino et al., 2010 [1]). Agar plate is small and portable while microfluidic device has machines and tubes connected to it. These advantages of agar plates may contribute to the intuitive understanding of clock hand movement, and lead to future applications (リンク貼る).
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<h3>The neuron analogy</h3>
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Table 1
 
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The clock device also contains a “control” reporter (amilGFP) for dual-reporter assay and a UV reset device (lambda cI repressor), which are explained later (ページの下のほうに飛ぶリンクを貼る).
 
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Figure 1 Multicellular Analog Clock
 
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<p class="ini">Our genetic “clock hand” , is similar in many ways to the action potential propagating along nerve cell axons (Fig. 3, Fig.4). Both can be classified as excitation waves which are driven to propagate in one direction by positive and negative feedback loops. Imagine a circle of axon. The propagation of action potential is just like a clock hand movement (Fig. 5). We explain the mechanism of our clock “gear” by analogy with action potential of the nerve cell.</p>
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Figure 2 The clock device. Pink box gene (luxI and luxR) forms the positive feedback loop of AHL production. Blue box (tetR, aiiA, and sRNAs) are the suppressors used for negative feedback loops. mCherry is the red reporter protein for visualization of the excitation wave (“clock hand”). amilGFP is the yellow reporter protein expressed constitutively as an internal standard.
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The neuron analogy
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<img src="https://static.igem.org/mediawiki/2013/9/91/Fig3yamada.png" class="figure">
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<p><b>Figure 3</b> The clock hand moves in one direction; from the past to the future.</p>
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Our genetic “clock hand” , is similar in many ways to the action potential propagating along nerve cell axons (Fig. 3, Fig.4). Both can be classified as excitation waves which are driven to propagate in one direction by positive and negative feedback loops. Imagine a circle of axon. The propagation of action potential is just like a clock hand movement (Fig. 5). We explain the mechanism of ourclock “gear” by analogy with action potential of the nerve cell.
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<img src="https://static.igem.org/mediawiki/2013/b/bb/Fig4yamadayamada.png" class="figure">
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<p><b>Figure 4</b> One directional propagation of action potential</p>
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Figure 3 The clock hand moves in one direction; from the past to the future.
 
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Figure 4 One directional propagation of action potential
 
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<p>When stimulated, a pulse of positive membrane potential (action potential) is generated. (Fig. 5). Positive membrane potential opens voltage-gated Na+ channel. Influx of Na+ increases positive membrane potential. This is positive feedback to amplify positive membrane potential. After a few milliseconds, positive membrane potential opens voltage-gated K+ channel. Increased K+ outflux decreases membrane potential. This is negative feedback to decrease positive membrane potential (Fig. 6).</p>
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When stimulated,, a pulse of positive membrane potential (action potentail) is generated. (Fig. 5). Positive membrane potential opens voltage-gated Na+ channel. Influx of Na+ increases positive.membran potential. This is positive feedback to amplify positive membrane potential. After a few milliseconds, positive membrane potential opens voltage-gated K+ channel. Increased K+ outflux decreases membrane potential. This is negative feedback to decreases positive membrane potential (Fig. 6).
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Figure 5 Action potential of nerve cell
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<img src="https://static.igem.org/mediawiki/2013/4/4d/Fig6yamada.png" class="figure">
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<p>Figure 5 Action potential of nerve cell</p>
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<img src="https://static.igem.org/mediawiki/2013/6/69/Genko_no_fig6.png" class="figure">
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Figure 6 Positive and negative feedback loops regulate positive membrane potential to drive the action potential along nerve cell axon.
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<p><b>Figure 6</b> Positive and negative feedback loops regulate positive membrane potential to drive the action potential along nerve cell axon.</p>
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The positive membrane potential spreads along axon, coupling membrane potential of neighboring region, and causes another rising phase and falling phase. This is observed as action potential propagating along a nerve cell axon.  
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<p>The positive membrane potential spreads along axon, coupling membrane potential of neighboring region, and causes another rising phase and falling phase. This is observed as action potential propagating along a nerve cell axon. </p>
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The feedback loops and coupling creates one-directional movement in nerve cell ..Nerve cell and our Clock have the positive feedback followed by negative feedback and coupling in common (Table 2). Thus, as in the nerve cell, the one directional excitation wave may be generated on our Clock (Fig. 7).
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<p>The feedback loops and coupling creates one-directional movement in nerve cell. Nerve cell and our Clock have the positive feedback followed by negative feedback and coupling in common (Table 2). Thus, as in a nerve cell, the one directional excitation wave may be generated on our Clock (Fig. 7).</p>
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Table 2 Action potential and clock hand
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<p><b>Table 2</b> Action potential and clock hand</p>
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  <img src="https://static.igem.org/mediawiki/2013/5/59/Tab2yamada.png" class="figure">
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  <br>
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Figure. 7
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<img src="https://static.igem.org/mediawiki/2013/e/e4/Fig6_2.png" class="figure">
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<p><b>Figure. 7</b></p>
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Positive feedback loop
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<h3>Positive feedback loop</h3>
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The positive feedback loop of the clock “gear” is composed of luxI (3OC6HSL(AHL) synthetase), the pLux/Tet hybrid promoter, and the luxR activator(Fig. 8).  
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<p class="ini">The positive feedback loop of the clock “gear” is composed of luxI (3OC6HSL(AHL) synthetase), the pLux/Tet hybrid promoter, and the luxR activator(Fig. 8). </p>
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<img src="https://static.igem.org/mediawiki/2013/1/12/Fig2_2_3yamada.png" class="figure">
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Fig. 8 Positive feedback loop
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<p><b>Fig. 8</b> Positive feedback loop</p>
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This positive feedback loop makes rising phase of gene expression. It works as follows (Fig. 9);
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<p>This positive feedback loop makes rising phase of gene expression. It works as follows (Fig. 9);<p>
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0, LuxR is constitutively expressed in cells.
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<p>0, LuxR is constitutively expressed in cells.</p>
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1, AHL flows in from out of the cell and binds to LuxR.
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<p>1, AHL flows in from out of the cell and binds to LuxR.</p>
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2, the AHL-LuxR complex binds to the pLux/Tet hybrid promoter and increase the transcription rate of genes including luxI.
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<p>2, the AHL-LuxR complex binds to the pLux/Tet hybrid promoter and increase the transcription rate of genes including luxI.</p>
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3, LuxI synthesizes AHL.
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<p>3, LuxI synthesizes AHL.</p>
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<img src="https://static.igem.org/mediawiki/2013/7/7b/Fig7yyyy.png" class="figure">
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Figure. 9 Positive feedback loop.
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<p><b>Figure. 9</b> Positive feedback loop.</p>
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This process occurs not only in a single cell. Intercellular positive feedback loops are formed and function as intercellular coupling like a spreading positive membrane potential of a nerve cell axon (Figure 10); AHL is synthesized in a cell and diffuse across cell membrane, and activate transcription in other cells. This cell-cell communication enables intercellular synchronization of gene expression, which drives the one directional movement of the “clock hand”.
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<p>This process occurs not only in a single cell. Intercellular positive feedback loops are formed and function as intercellular coupling like a spreading positive membrane potential of a nerve cell axon (Figure 10); AHL is synthesized in a cell and diffuses across cell membrane, and activates transcription in other cells. This cell-cell communication enables intercellular synchronization of gene expression, which drives the one directional movement of the “clock hand”.</p>
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<img src="https://static.igem.org/mediawiki/2013/1/13/1013Figure10_1.png" class="figure">
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<img src="https://static.igem.org/mediawiki/2013/5/5c/1013Figure10.png" class="figure">
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<img src="https://static.igem.org/mediawiki/2013/c/ca/1013Figure10_3.png" class="figure">
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<p><b>Figure 10</b> Intercellular coupling</p>
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<h3>Multi-Negative feedback system</h3>
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<p>Our clock “gear” has 4 negative feedback loops of different mechanisms (Table3), which are formed by tetR, aiiA, anti-mCherry sRNA, and anti-luxI sRNA (Fig. 11). All suppressors are regulated under the pLux/Tet promoter and expressed when stimulated by AHL. This negative feedback loops makes falling phase of gene expression.</p>
   
   
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Figure 10 Intercellular coupling
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<img src="https://static.igem.org/mediawiki/2013/1/13/Fig2_2_2yamada.png" class="figure">
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<p><b>Figure 11 </b>Multi-negative feedback system</p>
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<br>
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<p><b>Table 3</b> Multi-negative feedback system </p>
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<img src="https://static.igem.org/mediawiki/2013/2/2e/Tab1yamada.png" class="figure">
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Multi-Negative feedback system
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<p>TetR binds to pLux/tet and represses transcription of mCherry, luxI, and 4 suppressors including tetR. </p>
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Our clock “gear” has 4 negative feedback loops of different mechanisms (Table3), which are formed by tetR, aiiA, anti-mCherry sRNA, and anti-luxI sRNA (Fig. 11). All suppressors are regulated under the pLux/Tet promoter and expressed when stimulated by AHL. This negative feedback loops makes falling phase of gene expression.
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<p>AiiA degrades AHL in order to repress transcription indirectly.</p>
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<p>The sRNAs might work as a rapid suppressor for two reasons; 1. they repress the translation, which is the final step of prokaryotic gene expression, 2. they are quickly produced since they do not require time for translation of themselves. Both of sRNAs facilitate degradation of mRNA that contains mCherry, luxI, tetR, and aiiA. Anti-mCherry sRNA represses translation of mCherry gene, and anti-luxI does that of luxI gene. Repression of luxI causes decreased production of AHL, while repression of mCherry doesn’t affect AHL.</p>
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<p>The differences of mechanisms may lead to various patterns of suppression of various strength and time (Fig. 12). This multi-negative feedback system is a novel one which is not reported in previous papers or past iGEM projects. We designed 20 combinations of these 4 negative feedback loops and sought for the best combination for the Multicellular Analog Clock.</p>
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<p>Hasty group reported[1] the system with only one negative feedback loop of AiiA. Their system generated synchronized oscillation. This system shows bursts without any external stimuli. The burst is not suitable for the Analog Clock, because it means inappropriate addition of clock hands. TetR and 2 sRNAs were added in our clock device. These additional suppressors may lead to stronger repression and prevents burst of other clock hands.</p>
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Figure 11 Multi-negative feedback system
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Table 3 Multi-negative feedback system  
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<img src="https://static.igem.org/mediawiki/2013/d/d7/Fig9aysyshyh.png" class="figure">
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<p><b>Figure 12</b> Multi-negative feedback system.</p>
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TetR binds to pLux/tet and repress transcription of mCherry, luxI, and 4 suppressors including tetR.  
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<h3>The excitation wave</h3>
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AiiA degrades AHL to repress transcription indirectly.
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<p>Here, the propagation of excitation wave is explained concluding the discussion above .The positive feedback loop and Multi-negative feedback system drive the “clock hand”, the excitation wave of gene expression. The propagation of excitation wave is generated as follows (Fig. 13);</p>
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The sRNAs might work as rapid suppressor for two reasons; 1. they repress the translation, which is the final step of prokaryotic gene expression, 2. they are quickly produced since they do not require time for translation of themselves. Both of sRNAs facilitate degradation of mRNA that contains mCherry, luxI, tetR, and aiiA. anti-mCherry sRNA and anti-luxI sRNA repress translation of mCherry and luxI genes respectively. Repression of luxI causes decreased production of AHL, while repression of mCherry doesn’t affect AHL.
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The differences of mechanisms may lead to various patterns of suppression of various strength and time (Fig. 12). This multi-negative feedback system is a novel one which is not reported in previous papers or past iGEM projects. We designed 20 combinations of these 4 negative feedback loops and seek for the best combination for the Multicellular Analog Clock.
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<p>0. Cells are in the resting state. They are ready to respond to the stimulus by AHL.</p>
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<p>1. Cells are activated by AHL synthesized by neighbor cells and express mCherry. These cells are the “clock hand”.</p>
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<p>2. The LuxI is also expressed at the same time and synthesize AHL to activate neighbor cells.</p>
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<p>3. The supressors are also expressed to make unresponsive state, which prevents “clock hand” go backwards.</p>
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Hasty group reported[1] the system with only one negative feedback loop of AiiA. Their system generated synchronized oscillation. This system shows bursts without any external stimulus. The burst is not suitable for the Analog Clock, because it means inappropriate addition of a clock hand. TetR and 2 sRNAs were added in our clock device. These additional suppressors may lead to stronger repression and prevents burst of another clock hand.
 
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<img src="https://static.igem.org/mediawiki/2013/f/fd/1013Figure13.png" class="figure">
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Figure 12 Multi-negative feedback system.
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<p><b>Figure 13</b></p>
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The excitation wave
 
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Here, the propagation of excitation wave is explained concluding the discussion above .The positive feedback loop and Multi-negative feedback system drive the “clock hand”, the excitation wave of gene expression. The propagation of excitation wave is generated as follows (Fig. 13);
 
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0. Cells are in the resting state. They are ready to respond to the stimulus by AHL.
 
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1. Cells are activated by AHL synthesized by neighbor cells and express mCherry. These cells are the “clock hand”.
 
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2. The LuxI is also expressed at the same time and synthesize AHL to activate neighbor cells.
 
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3. The supressors are also expressed to make unresponsive state, which prevents “clock hand” go backwards.
 
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Figure 13
 
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Clock hand (visualization for naked eyes)
 
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The clock device has 2 reporter genes for dual-reporter assay, which allows accurate measurement of gene expression intensities.
 
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mCherry is a red reporter protein for the “clock hand”. mCherry expression intensity changes dynamically; is increased by AHL stimulus and decreased by 4 suppressors.
 
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amilGFP is yellow “control” reporter protein for ratiometric measurement with mCherry. amilGFP is constitutively expressed independently from clock “gear”.
 
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Without “control” reporter, background noises (changing agar plate environment surrounding cells, amount of resources for gene expression, cell population density, degradation tag activity, etc.) might obscure the dynamic gene expression of interest (=“clock hand”). Comparing the mCherry expression with amilGFP, you can clearly track the “clock hand” for a long time and will notice the unimportant noises disturbing the clock.
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<h3>Clock hand (visualization for naked eyes)</h3>
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<p>The clock device has 2 reporter genes for dual-reporter assay, which allows accurate measurement of gene expression intensities.</p>
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mCherry and amilGFP have strong color, which is visible to naked eyes under natural room light (Fig. 14). You can easily see the gene expression without special equipment.
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<p>mCherry is a red reporter protein for the “clock hand”. mCherry expression intensity changes dynamically, being increased by AHL stimulus and decreased by 4 suppressors.</p>
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<p>amilGFP is yellow “control” reporter protein for ratiometric measurement with mCherry. amilGFP is constitutively expressed independently from clock “gear”. </p>
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<p>Without “control” reporter, background noises (changing agar plate environment surrounding cells, amount of resources for gene expression, cell population density, degradation tag activity, etc.) might obscure the dynamic gene expression of interest (=“clock hand”). Comparing the mCherry expression with amilGFP, you can clearly track the “clock hand” for a long time and will notice the unimportant noises disturbing the clock.</p>
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Figure 14 strong color of mCherry and amilGFP (実験室で撮った写真を使う)
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<p>mCherry and amilGFP have strong color, which is visible to naked eyes under natural room light (Fig. 14). You can easily see the gene expression without special equipment.</p>
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Also, both of them are fluorescent protein and have different excitation and emission spectra. Measuring fluorescence (e.g. fluorescent microscopy, flow cytometry, …) will be a good way to measure the gene expression accurately. You can measure both fluorescence and color under natural light with one BioBrick device.
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<p>Also, both of them are fluorescent protein and have different excitation and emission spectra. Measuring fluorescence (e.g. fluorescent microscopy, flow cytometry, …) will be a good way to measure the gene expression accurately. You can measure both fluorescence and color under natural light with one BioBrick device.</p>
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amilGFP with LVA degradation tag is a new BioBrick part of our design. This BioBrick part will be used as a good reporter for its strong yellow color, fluorescence, and short lifetime, which enables intuitive understanding and real-time measurement of gene expression at the same time.
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<p>amilGFP with LVA degradation tag is a new BioBrick part of our design. This BioBrick part will be used as a good reporter for its strong yellow color, fluorescence, and short lifetime, which enables intuitive understanding and real-time measurement of gene expression at the same time.</p>
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LVA degradation tag
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<h4>LVA degradation tag</h4>
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C-termini of almost all the proteins in the clock device (luxI, tetR, aiiA, mCherry, and amilGFP) have LVA degradation tag for rapid degradation [2]. Rapid degradation of components of feedback loops may cause rapid change of gene expression, which  
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<p>C-termini of almost all the proteins in the clock device (luxI, tetR, aiiA, mCherry, and amilGFP) have LVA degradation tag for rapid degradation [2]. Rapid degradation of components of feedback loops may cause rapid change of gene expression, which  
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keeps “clock hand” small. Reporter genes should have short half-lives to visualize real-time changes of gene expression. LuxR has no degradation tag, since clock device needs constant expression of LuxR.
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keeps “clock hand” small. Reporter genes should have short half-lives to visualize real-time changes of gene expression. LuxR has no degradation tag, since clock device needs constant expression of LuxR.</p>
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UV reset device
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<h3>UV reset device</h3>
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Feedback loops of genetic circuit are not sufficient to generate one directional movement of mCherry “clock hand” along an E. coli circle. If you had only feedback loops, after adding AHL at one point of the circle to start the Clock, you will get two-way movement, which will soon disappear by collision of two “clock hands”.  
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<p>Feedback loops of genetic circuit are not sufficient to generate one directional movement of mCherry “clock hand” along an <i>E. coli</i> circle. If you had only feedback loops, after adding AHL at one point of the circle to start the Clock, you will get two-way movement, which will soon disappear by collision of two “clock hands”. </p>
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To turn this two-way movement to one-way, we need to make some part of a circle unresponsive before starting the clock. UV reset device was included in the clock device for this purpose. UV reset device contains UV sensor (UV-susceptible cI repressor and the phage lambda PR promoter and suppressors (Fig. 15). )
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<p>To turn this two-way movement to one-way, we need to make some part of a circle unresponsive before starting the clock. UV reset device was included in the clock device for this purpose. UV reset device contains UV sensor (UV-susceptible cI repressor and the phage lambda PR promoter and suppressors (Fig. 15). )</p>
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<img src="https://static.igem.org/mediawiki/2013/3/31/Fig2_2_1yamada.png" class="figure">
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Figure 15
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<p><b>Figure 15</b></p>
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UV reset device make the “unresponsive period” when exposed to UV. The detailed mechanism is as follows (Fig. 16)
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<p>UV reset device makes the “unresponsive period” in response to exposure to UV. The detailed mechanism is as follows (Fig. 16)</p>
   
   
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0, cI repressors are constitutively expressed.
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<p>0, cI repressors are constitutively expressed.</p>
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1, UV induces cleavage (inactivation) of cI repressor.
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<p>1, UV induces cleavage (inactivation) of cI repressor.</p>
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2, phage lambda PR promoter is derepressed.
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<p>2, phage lambda PR promoter is derepressed.</p>
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3, suppressors are expressed, which makes unresponsive period.
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<p>3, suppressors are expressed, which makes unresponsive period.</p>
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<img src="https://static.igem.org/mediawiki/2013/3/38/Figure16_1.png" class="figure">
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Figure 16 UV reset device. UV induces suppressors to make unresponsive state.
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<img src="https://static.igem.org/mediawiki/2013/8/87/1013Figure16_2.png" class="figure">
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<img src="https://static.igem.org/mediawiki/2013/3/35/1013Figure16_3.png" class="figure">
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<p><b>Figure 16</b> UV reset device. UV induces suppressors to make unresponsive state.</p>
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UV treatment induces unresponsive period and AHL generate clock hand. One-way movement is generated when UV treatment was next to the point of AHL induction when you start the clock (Fig. 17).
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<p>UV treatment induces unresponsive period and AHL generate clock hand. One-way movement is generated when UV treatment was next to the point of AHL induction when you start the clock (Fig. 17).</p>
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<img src="https://static.igem.org/mediawiki/2013/6/6a/1013Figure17.png" class="figure">
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Figure 17 Start of the Multicellular Analog Clock. UV induction is just next to AHL.
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<p><b>Figure 17</b> Start of the Multicellular Analog Clock. UV induction is just next to AHL.</p>
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UV-susceptible cI repressors (E233K) were used in toggle switch of iGEM MIT 2010. However, MIT 2010[3] made only the composite toggle parts. Thus, we made a new BioBrick part of single UV-susceptible cI repressor (E233K) through site-directed mutagenesis. This standard basic part will be useful for future registry members.
+
<p>UV-susceptible cI repressors (E233K) were used in toggle switch of iGEM MIT 2010. However, MIT 2010[3] made only the composite toggle parts. Thus, we made a new BioBrick part of single UV-susceptible cI repressor (E233K) through site-directed mutagenesis. This standard basic part will be useful for future registry members.</p>
-
The full system
+
<h3>The full system</h3>
-
“Gear”, “Clock hand”, and UV reset device makes the full genetic circuit (BBa_K1124100) (Fig. 18).The circle of E. coli population on agar plate with BBa_K1124100 works as the Multicellular Analog Clock.
+
<p>“Gear”, “Clock hand”, and UV reset device make the full genetic circuit (<a href="http://parts.igem.org/Part:BBa_K1124100">BBa_K1124100</a><br>) (Fig. 18).The circle of <i>E. coli</i> population on agar plate with BBa_K1124100 works as the Multicellular Analog Clock.</p>
-
+
<img src="https://static.igem.org/mediawiki/2013/d/de/Fig2_2yamada.png" class="figure">
-
Figure 18 The full system
+
<p><b>Figure 18</b></p>
                      
                      
-
            <h2 id="mBackground">Background</h2>
+
 
-
                 
+
<h2 id="mApplicaton">Applicaton</h2>
-
           
+
<p>In this project, we constructed an analog clock of <i>E.coli</i> for which quorum sensing was utilized. The system is simple because it has only one hand. So other functions can be easily adopted. For example, if particular smell or light is given out every regular time, it becomes an alarm clock. Some past iGEM projects such as MIT2006 and NCBS2009 referred to how to produce scent. In addition, the fine adjustment device could be constructed which is based on the velocity of the hand of it changing by external input. For instance, it is possible to precisely control the clock device by employing promoterAHL which promotes transcription according to the density of AHL and constructing the sequences, such as promoterAHL-luxI and promoterAHL-(tetR, aiiA, sRNAs). While UV reset device resets the clock by decomposition of c1 repressor (E233K), it resets the clock by increase or decrease in AHL.                      </p> 
-
            <p class="ini">To simulate the cell-cell communication system, we developed a delayed differential equation model. The equation used in the model are followings. The variables are described in the following table. </p>
+
<p>As another use of clock device, there is a quantity measuring device. The outline is as follows. First, we move the hand by means of the clock device, and then stop the movement of it after a fixed period of time by such as irradiation of UV. As a result, quantity can be visibly measured. For example, we could measure the density of a material by using this device.  The material to be measured defines “S”, we make a construction, promoterS-RBS-luxI-d.term-(clock-device)-(UV reset device). PromoterS promotes transcription in response to the existence of S. Then, because the velocity of the hand changes according to the density of S, we could conclude that if it swung longer, the density is higher and that if it swung shorter, the density is lower.(Fig.1)  </p>
-
            <img src="ddes.png" class="figure">
+
                          <img src="https://static.igem.org/mediawiki/2013/9/9a/Fig1irie2.png" clacc="figure">
 +
  <p>Fig1. The density of a material measuring device                      </p>
-
<p> In our cell-cell communication system, the major kinetic events are: mCherry synthesis and degradation, LuxI synthesis and degradation, TetR synthesis and degradation, AHL synthesis and degradation. These kinetic events are contained in the equations. The following describes how the equations are developed.
 
-
</p>
 
-
<ul>
+
<p>Not only whole clock device, but also the parts we made for this project constitutive of it, such as amilGFP and UV sensor, could be applied to different purposes. amilGFP with LVA degradation tag gives out strong yellow light under natural light and degrades rapidly. Using these features enables us to measure visually, depending on the flow of time. Also, UV sensor can express any gene in any time by only irradiating UV.    </p>
-
<li>mCherry synthesis and degradation
+
-
<li>LuxI synthesis and degradation</li>
+
-
<li>TetR synthesis and degradation</li>
+
-
<li>AHL synthesis and degradation</li>
+
-
</ul>
+
-
 
+
-
<h2 id="mSystems">Systems</h2>
+
-
<h2 id="mResults_and_Discussion">Results and Discussion</h2>
+
-
<h2 id="mApplicaton">Applicaton</h2>
+
-
<p>In this project, we constructed an analog clock of E.coli for which quorum sensing was utilized. The system is simple because it has only one hand. So other functions can be easily adopted. For example, if particular smell or light is given out every regular time, it becomes an alarm clock. Some past iGEM projects such as MIT2006 and NCBS2009 referred to how to produce scent. In addition, the fine adjustment device could be constructed which is based on the velocity of the hand of it changed by external input. For instance, it is possible to precisely control the clock device by employing promoterAHL which promotes transcription according to the density of AHL and constructing the sequences, such as promoterAHL-luxI and promoterAHL-(tetR, aiiA, sRNAs). While UV reset device resets the clock by decomposition of c1 repressor (E234K), it resets the clock by increase or decrease in AHL.                      </p> 
+
-
<p>As other use of clock device, there is a quantity measuring device. The outline is as follows. First, we move the hand by means of the clock device, and then stop the movement of it after a fixed period of time by such as irradiation of UV. As a result, quantity can be visibly measured. For example, we could measure the density of a material by using this device.  The material to be measured defines “S”, we make the sequences, promoterS-RBS-luxI-d.term-(clock-device)-(UV reset device). PromoterS promotes transcription because of the existence of S. Then, because the velocity of the hand changes according to the density of S, we could conclude that if it swung longer, the density is higher and that if it swung shorter, the density is lower. (Fig.1)      </p>
+
-
                         
+
-
 
+
-
<p>Fig1. The density of a material measuring device                      </p>
+
-
 
+
-
<p>Not only whole clock device, but also the parts we made for this project constitutive of it, such as amilGFP and UV sensor, could be applied to different purposes. amilGFP with LVA degradation tag gives out strong yellow light by natural light and degrades rapidly. Using these features enables us to measure visually, depending on the flow of time. Also, UV sensor can express any gene in any time by only irradiating UV.    </p>
+
<p>As stated above, the clock device and its parts can be used for various purposes and these applications would also give synthesis biology great progress.              </p>
<p>As stated above, the clock device and its parts can be used for various purposes and these applications would also give synthesis biology great progress.              </p>
             <h1 id="RNA_Silencing">RNA Silencing</h1>
             <h1 id="RNA_Silencing">RNA Silencing</h1>
-
             <h2 id="mSummary">Summary</h2>
+
             <h2 id="rBackground">Background</h2>
-
            <p class="ini"> Our concept of the multicellular analog clock is based on qualitative assumption such as how negative feedback loop behaves, how AHL diffuses, and so on. To ascertain our multicellular analog clock can function as an analogue clock, namely, to confirm the feasibility of our cell-cell communication included gene circuit, and to deepen understanding of behavior of the system, we conducted the following simulation. </p>
+
-
 
+
-
            <p>Our model for multicellular analog clock consists of four parts: DDE analysis, parameter sensitivity analysis, parameter sweep, stochastic analysis. DDE analysis is to examine the feasibility of our project, and also provides the foundation for the other parts of analysis. Through parameter sensitivity analysis, we gained more insight of the relationship between input and output variables. The insight led to the third part of analysis, in which parameter sweep enabled us to grasp appropriate ranges fo the identified sensitive parameters. Finally, we conducted stochastic analysis with the sensitive parameters fixed, and simulate our device's behavior under the actual conditions.
+
-
            </p>
+
-
                   
+
-
            <h2 id="mBackground">Background</h2>
+
                    
                    
             <p class="ini"><b>Introduction</b></p>
             <p class="ini"><b>Introduction</b></p>
-
             <p class="ini">It is needless to say that gene knockdown is important in the study of biology. Genomic knockdown by making the target gene mutate requires much labor and time. In eucaryotes, RNAi has contributed to the functional analysis of genes. E.coli, which belongs to procaryotes, does not have the mechanism needed for RNAi and this method is not usually used for E.coli.</p>
+
             <p class="ini">It is needless to say that gene knockout is important in the study of biology. Genomic knockout by making the target gene mutate requires much labor and time. In eucaryotes, RNAi has contributed to the functional analysis of genes. <i>E. coli</i>, which belongs to procaryotes, does not have the mechanism needed for RNAi and this method is not usually used for <i>E. coli</i>.</p>
-
             <p>But in recent years it has been becoming clear that E.coli has the mechanism of gene silencing by small regulatory RNAs (sRNA) which have complementary sequences with their target mRNAs [1].We used the mechanism for gene knockdown by designing synthetic sRNAs and inducing plasmids coding them into bacterial cells. This method can be done by gene introduction and enables us to knock down genes by combining our new BioBrick parts "micC scaffold" and complementary sequences with the target gene [2].</p>
+
             <p>But in recent years it has been becoming clear that <i>E. coli</i> has the mechanism of gene silencing by small regulatory RNAs (sRNA) which have complementary sequences with their target mRNAs [1].We used the mechanism for gene knockout by designing synthetic sRNAs and inducing plasmids coding them into bacterial cells. This method can be done by gene introduction and enables us to knock down genes by combining our new BioBrick parts "micC scaffold" and complementary sequences with the target gene [2].</p>
<p>We tested using our BioBrick parts by applying them for the following objectives.</p>
<p>We tested using our BioBrick parts by applying them for the following objectives.</p>
<p><ul>
<p><ul>
<li>Acceleration of Biosynthesis of L-DOPA, a substance utilized for treatment of Parkinson’s disease.</li>
<li>Acceleration of Biosynthesis of L-DOPA, a substance utilized for treatment of Parkinson’s disease.</li>
-
<li>Improvement of H2 Productive Capacity of E.coli</li>
+
<li>Improvement of H2 Productive Capacity of <i>E. coli</i></li>
<li>Suppressor of Multi-Negative Feedback System of Clock Device</li>
<li>Suppressor of Multi-Negative Feedback System of Clock Device</li>
</ul></p>
</ul></p>
-
<h2 id="sSystem">System</h2>
+
<h2 id="rSystem">System</h2>
<p class="ini"><b>Mechanism of RNA silencing in Escherichia coli</b></p>
<p class="ini"><b>Mechanism of RNA silencing in Escherichia coli</b></p>
<p class="ini">
<p class="ini">
-
Escherichia coli have several trans-acting sRNA molecules repressing the expressions of their target genes, such as micC and sgrS and so on. Their mechanism has been becoming clear in recent years. (Fig.1) These sRNA molecules consint of complementary sequences to their target mRNAs and a scaffold sequence that recruites the Hfq protein. Hfq protein, one of the RNA chaperones, facilitates the hybridization of sRNAs to mRNA by their complementary sequences and with RNase E. RNase E causes the degradation of the sRNAs by the target mRNAs. So the mechanism of gene repression by Hfq-dependent sRNAs is composed of the translational repression by their complementary sequences and Hfq proteins and the degradation of the target mRNAs by RNase E [3].</p>
+
Escherichia coli have several trans-acting sRNA molecules repressing the expressions of their target genes, such as micC and sgrS and so on. Their mechanism has been becoming clear in recent years. (Fig.1) These sRNA molecules consist of complementary sequences to their target mRNAs and a scaffold sequence that recruites the Hfq protein. Hfq protein, one of the RNA chaperones, facilitates the hybridization of sRNAs to mRNA by their complementary sequences and with RNase E. RNase E causes the degradation of the sRNAs by the target mRNAs. So the mechanism of gene repression by Hfq-dependent sRNAs is composed of the translational repression by their complementary sequences and Hfq proteins and the degradation of the target mRNAs by RNase E [3].</p>
<p>We tried to apply the Hfq protein-dependent RNA silencing for several objectives by establishing the design principle of sRNAs.</p>
<p>We tried to apply the Hfq protein-dependent RNA silencing for several objectives by establishing the design principle of sRNAs.</p>
<img src="https://static.igem.org/mediawiki/2013/c/c3/Yamada_fig1_2.png" class="figure">
<img src="https://static.igem.org/mediawiki/2013/c/c3/Yamada_fig1_2.png" class="figure">
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<br>
<br>
<p>In order to design Hfq protein-dependent sRNAs simply, we used the following design principle. Our sRNAs are composed of two regions: a target-binding sequence and a scaffold sequence. We made the sRNA generator by combining these with a promoter and a terminator. (Fig.2)</p>
<p>In order to design Hfq protein-dependent sRNAs simply, we used the following design principle. Our sRNAs are composed of two regions: a target-binding sequence and a scaffold sequence. We made the sRNA generator by combining these with a promoter and a terminator. (Fig.2)</p>
-
<p>We chose a region of the target sequence that spans the AUG to nucleotide +21 of the mRNA as the binding site. This was because a previous study suggested that it is effective especially to select the region overlapping the TIR (Translation Initiation Region) of the mRNA [2]. The scaffold sequence recruits the Hfq protein, which facilitates the hybridization of sRNA and target mRNA as well as mRNA degradation. Of the scaffold sequences of E.coli, we selected MicC because of the superior repression capability [2].</p>
+
<p>We chose a region of the target sequence that spans from the AUG to nucleotide +21 of the mRNA as the binding site. This was because a previous study suggested that it is effective especially to select the region overlapping the TIR (Translation Initiation Region) of the mRNA [2]. The scaffold sequence recruits the Hfq protein, which facilitates the hybridization of sRNA and target mRNA as well as translational inhibition. Among the scaffold sequences of <i>E. coli</i>, we selected MicC because of the superior repression capability and made the scaffold BioBrick part (BBa_K1124005). In this design, there is an unnecessary sequence, which is caused by using as BioBrick parts, between the promoter and the target-binding sequence.</p>
 +
<p>We proved that the MicC scaffold and our sRNA design with pConst-sRNA(anti-tyrR) functions by qRT-PCR.</p>
<img src="https://static.igem.org/mediawiki/2013/3/3c/Yamada_1ab_.png" class="figure">
<img src="https://static.igem.org/mediawiki/2013/3/3c/Yamada_1ab_.png" class="figure">
<p><b>Fig.2</b> Design principle of our synthetic sRNAs</p>
<p><b>Fig.2</b> Design principle of our synthetic sRNAs</p>
<br>
<br>
<p class="ini"><b>Automatic RNA silencing primer designer</b></p>
<p class="ini"><b>Automatic RNA silencing primer designer</b></p>
-
<p class="ini">Here we present gAutomatic RNA silencing primer designerh. You can get a pair of primer to generate sRNA sufficient to knockdown a gene of your choice.</p>
+
<p class="ini">Here we present Automatic RNA silencing primer designer. You can get a pair of primer to generate sRNA sufficient to knockdown the gene of your choice.</p>
-
<p><i>Program 1</i></p>
+
 
 +
<h3><i>Program 1</i></h3>
<p>When you perform PCR with primers designed by Program 1, you will get the PCR product like the diagram below.</p>
<p>When you perform PCR with primers designed by Program 1, you will get the PCR product like the diagram below.</p>
 +
<img src="https://static.igem.org/mediawiki/2013/0/02/Minakuchi.png"  class="figure">
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<div>
<div>
-
<h3>CDS</h3>
+
<b>CDS</b>
<p id="sequence"></p>
<p id="sequence"></p>
-
<h3>Forward Primer for BioBrick</h3>
+
<b>Forward Primer for BioBrick</b>
<p id="generated"></p>
<p id="generated"></p>
</div>
</div>
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<p>4. Perform PCR using primers given by the program. The template must be <a href="http://parts.igem.org/Part:BBa_K1124112">BBa_K1124112</a> on the plasmid backbone pSB1C3, pSB1A3, or pSB1AK3.</p>
<p>4. Perform PCR using primers given by the program. The template must be <a href="http://parts.igem.org/Part:BBa_K1124112">BBa_K1124112</a> on the plasmid backbone pSB1C3, pSB1A3, or pSB1AK3.</p>
<p>5. Cut the PCR product by Xba1 and Pst1, and ligate it with backbone.</p>
<p>5. Cut the PCR product by Xba1 and Pst1, and ligate it with backbone.</p>
-
<p>6. (Alternative to Procedure 5) Cut the PCR product by Xba1 and Pst1, and the promoter part on a plasmid backbone by Spe1 and Pst1. Ligate these digests and you will get the full constructed part that expresses sRNA sufficient to knockdown a gene of your choice.</p>
+
<p>6. (Alternative to Procedure 5) Cut the PCR product by Xba1 and Pst1, and the promoter part on a plasmid backbone by Spe1 and Pst1. Ligate these digests and you will get the full constructed part that expresses sRNA sufficient to knockdown the gene of your choice.</p>
-
<p>Program 2</p>
+
<h3><i>Program 2</i></h3>
<p>
<p>
When you perform PCR with primers designed by Program 2 using BBa_K1124123.as the template, you will get the PCR product like the diagram below.</p>
When you perform PCR with primers designed by Program 2 using BBa_K1124123.as the template, you will get the PCR product like the diagram below.</p>
 +
 +
<img src="https://static.igem.org/mediawiki/2013/7/76/Minakuchi_2.png"  class="figure" height="200">
-
<p>The PCR product is the full constructed part that expresses sRNA sufficient to knockdown a gene of your choice.
+
<p>The PCR product is the full constructed part that expresses sRNA sufficient to knockdown the gene of your choice. You never have to digest and ligate the plasmid before <i><i>E. coli</i></i> transformation.
</p>
</p>
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<div>
<div>
-
<h3>CDS</h3>
+
<b>CDS</b>
<p id="sequence2"></p>
<p id="sequence2"></p>
-
<h3>Forward Primer for Inverse PCR</h3>
+
<b>Forward Primer for Inverse PCR</b>
<p id="fp"></p>
<p id="fp"></p>
-
<h3>Reverse Primer for Inverse PCR</h3>
+
<b>Reverse Primer for Inverse PCR</b>
<p id="rp"></p>
<p id="rp"></p>
</div>
</div>
Line 422: Line 403:
<p>1. Input the first 24nt of the CDS of the gene you would like to knockdown.</p>
<p>1. Input the first 24nt of the CDS of the gene you would like to knockdown.</p>
-
<p>2. The sequences of forward primer and reverse primer are shown.</p>
+
<p>2. The sequences of forward primer and the reverse primer are shown.</p>
<p>3. Perform inverse PCR with using primers given by the program. The template is <a href="http://parts.igem.org/Part:BBa_K1124123">BBa_K1124123</a>. There is no restriction on the plasmid backbone. You must use DNA polymerase with no displacement activity nor 5f¨3fexonuclease activity.</p>
<p>3. Perform inverse PCR with using primers given by the program. The template is <a href="http://parts.igem.org/Part:BBa_K1124123">BBa_K1124123</a>. There is no restriction on the plasmid backbone. You must use DNA polymerase with no displacement activity nor 5f¨3fexonuclease activity.</p>
-
<p>4. The PCR product is a plasmid with nicks. Transform E. coli with the PCR product and the plasmid will expresses sRNA sufficient to knockdown a gene of your choice.</p>
+
<p>4. The PCR product is a plasmid with nicks. Transform <i>E. coli</i> with the PCR product and the plasmid will expresses sRNA sufficient to knockdown the gene of your choice.</p>
<p></p>
<p></p>
Line 434: Line 415:
-
<h2 id="mResults_and_Discussion">Application</h2>
+
<h2 id="rResults_and_Discussion">Application</h2>
<p class="ini"><b>Acceleration of Biosynthesis of L-DOPA</b></p>
<p class="ini"><b>Acceleration of Biosynthesis of L-DOPA</b></p>
-
<p class="ini">n order to make E.coli produce L-DOPA, we made hpaBC (encoding the enzyme 4-hydroxyphenylacetate 3-hydroxylase) BioBrick parts by cloning it from E.coli BL21 strain. HpaBC catalyzes the hydroxylation of tyrosine and produces L-DOPA (Fig.3a). But the production rate of L-DOPA was low. Then we designed sRNAs knocking down several genes involved in the pathway from glucose to L-Tyr in order to increase the carbon flow to tyrosine synthetic pathway, because the amount is thought to be dependent to that of L-Tyr, the precursor of L-DOPA [4].</p>
+
<p class="ini">In order to make <i>E. coli</i> produce L-DOPA, we made hpaBC (encoding the enzyme 4-hydroxyphenylacetate 3-hydroxylase) BioBrick parts by cloning it from <i>E. coli</i> BL21 strain. HpaBC catalyzes the hydroxylation of tyrosine and produces L-DOPA (Fig.3a). But the production rate of L-DOPA was low. Then we designed sRNAs knocking down several genes involved in the pathway from glucose to L-Tyr in order to increase the carbon flow to tyrosine synthetic pathway, because the amount is thought to be dependent on that of L-Tyr, the precursor of L-DOPA [4].</p>
-
<p>We selected tyrR(encoding tyrosine repressor), csrA(encoding carbon-storage regulator, which regulates the expression of enzyme genes involved in glycolysis) and tyrC (phosphoribosylanthranilate isomerase) as the targets [2]. tyrR and csrA have been shown to promote L-Tyr biosynthesis in a previous study.(Fig.3b)</p>
+
<p>We selected tyrR(encoding tyrosine repressor), csrA(encoding carbon-storage regulator, which regulates the expression of enzyme genes involved in glycolysis) and tyrC (phosphoribosylanthranilate isomerase) as the targets [2]. tyrR and csrA have been shown to promote L-Tyr biosynthesis in the previous study.(Fig.3b)</p>
-
<p>Because these sRNA are thought to damage the growth of E.coli, we tried several promoters having different strength. </p>
+
<p>Because these sRNAs are thought to damage the growth of <i>E. coli</i>, we tried several promoters having different strength. </p>
<img src="https://static.igem.org/mediawiki/2013/b/bc/L-DOPA.png" class="figure">
<img src="https://static.igem.org/mediawiki/2013/b/bc/L-DOPA.png" class="figure">
<p><b>Fig.3a</b> Hydroxilation of tyrosine to L-DOPA by HpaBC</p>
<p><b>Fig.3a</b> Hydroxilation of tyrosine to L-DOPA by HpaBC</p>
<img src="https://static.igem.org/mediawiki/2013/1/1a/Yamada_fig2.png" class="figure">
<img src="https://static.igem.org/mediawiki/2013/1/1a/Yamada_fig2.png" class="figure">
-
<p><b>Fig.3b</b> The tyrosine biosynthetic pathway in E.coli and our metabolic engineering strategy</p>
+
<p><b>Fig.3b</b> The tyrosine biosynthetic pathway in <i>E. coli</i> and our metabolic engineering strategy</p>
<br>
<br>
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-
<p class="ini"><b>Improvement of H2 Productive Capacity of E.coli</b></p>
+
<p class="ini"><b>Improvement of H2 Productive Capacity of E. coli</b></p>
-
<p class="ini">In 2012, we UT-Tokyo made "H2 E.coli", which produces more H2 than usual E.coli. This year we tried to improve its H2 productive capacity further by designing anti-hycA. </p>
+
<p class="ini">In 2012, we UT-Tokyo made "H2 <i>E. coli</i>", which produces more H2 than usual <i>E. coli</i>. This year we tried to improve its H2 productive capacity further by designing anti-hycA. </p>
-
<p>E.coli naturally produces hydrogen through a process called mixed acid fermentation (MAF pathway) (Fig.4a). HycA represses the transcription of the proteins of FHL complex, which produces H2 from formate. </p>
+
<p><i>E. coli</i> naturally produces hydrogen through a process called mixed acid fermentation (MAF pathway) (Fig.4a). HycA represses the transcription of the proteins of FHL complex, which produces H2 from formate. </p>
-
<p>Last year we tried to derepress it by introducing plasmids including many decoy binding sites in our project "Inhibition without knockdown". This method has several methods. It allows to knockdown by introducing BioBrick parts and does not make scars on the candidate's genome. But it can be used only for DNA binding proteins and the strength cannot be regulated without changing the plasmid.</p>
+
<p>Last year we tried to derepress it by introducing plasmids including many decoy binding sites in our project "Inhibition without knockout". This method has several methods. It allows to knockdown by introducing BioBrick parts and does not make scars on the candidate's genome. But it can be used only for DNA binding proteins and the strength cannot be regulated without changing the plasmid.</p>
-
<p>Though the merit of "Inhibition without knockdown" is retained in knockdown with Hfq protein-dependent sRNAs, our new method does not have these demerits.</p>
+
<p>Though the merit of "Inhibition without knockout" is retained in knockdown with Hfq protein-dependent sRNAs, our new method does not have these demerits.</p>
<img src="https://static.igem.org/mediawiki/2013/c/c0/Yamada_3.png" class="figure">
<img src="https://static.igem.org/mediawiki/2013/c/c0/Yamada_3.png" class="figure">
<p><b>Fig.4a</b> The function of FHL complex and HycA</p>
<p><b>Fig.4a</b> The function of FHL complex and HycA</p>
-
<img src="https://static.igem.org/mediawiki/2013/b/be/Yamada_4.png class="figure" width="400">
+
<img src="https://static.igem.org/mediawiki/2013/b/be/Yamada_4.png" class="figure" width="400">
<p><b>Fig.4b</b> The system of "Inhibition without knockout"</p>
<p><b>Fig.4b</b> The system of "Inhibition without knockout"</p>
<p>By introducing plasmids with many decoy binding sites of the target proteins, we tried to prevent repressors from acting on genomic sites.</p>
<p>By introducing plasmids with many decoy binding sites of the target proteins, we tried to prevent repressors from acting on genomic sites.</p>
Line 462: Line 443:
<p class="ini"><b>Suppressor of Multi-Negative Feedback System of Clock Devise</b></p>
<p class="ini"><b>Suppressor of Multi-Negative Feedback System of Clock Devise</b></p>
<p class="ini">In our project, Multicellular Analog Clock, we needed a suppressor to be used for the Multi-Negative Feedback System and UV reset devise. Our clock devise uses novel systems which are not seen in previous papers or past iGEM project and it is difficult to say what kind of suppressors is best unless we actually try them. So we designed two kinds of sRNAs that bind to the TIR of mCherry and luxI as the suppressors (anti-mCherry and anti-luxI).</p>
<p class="ini">In our project, Multicellular Analog Clock, we needed a suppressor to be used for the Multi-Negative Feedback System and UV reset devise. Our clock devise uses novel systems which are not seen in previous papers or past iGEM project and it is difficult to say what kind of suppressors is best unless we actually try them. So we designed two kinds of sRNAs that bind to the TIR of mCherry and luxI as the suppressors (anti-mCherry and anti-luxI).</p>
-
<p>sRNA suppressors will behave differently from AiiA and TetR. sRNAs express and decompose more rapidly than these protein based suppresors and act on different targets.</p>
+
<p>sRNA suppressors will behave differently from AiiA and TetR. sRNAs express and decompose more rapidly than suppresors based on these proteins and act on different targets.</p>
<p>The two sRNAs are act on different molecules within the clock pathway and therefore expected to influence clock behavior differently. Translational inhibition is caused only by sRNAs binding to the TIR of the target gene while degradation by RNase E is caused by any sRNAs binding to the mRNA.</p>
<p>The two sRNAs are act on different molecules within the clock pathway and therefore expected to influence clock behavior differently. Translational inhibition is caused only by sRNAs binding to the TIR of the target gene while degradation by RNase E is caused by any sRNAs binding to the mRNA.</p>
<p>The differences of mechanisms may lead to various patterns of suppression of various strength and time.</p>
<p>The differences of mechanisms may lead to various patterns of suppression of various strength and time.</p>
-
<h2 id="sReferences">References</h2>
+
<h2 id="rReferences">References</h2>
<p class="ini">[1]Aiba, H. (2007). Mechanism of RNA silencing by Hfq-binding small RNAs. Current opinion in microbiology, 10(2), 134-139.</p>
<p class="ini">[1]Aiba, H. (2007). Mechanism of RNA silencing by Hfq-binding small RNAs. Current opinion in microbiology, 10(2), 134-139.</p>
<p class="ini">[2]Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.</p>
<p class="ini">[2]Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.</p>

Latest revision as of 05:40, 13 October 2014

           PROJECT
       

Multicellular Analog Clock

Systems

What is the Multicellular Analog Clock?

The goal of the project is to make the Multicellular Analog Clock which has following features;

1. One directional movement of position of gene expression on a multicellular scale (like the movement of clock hand).

2. Easy observation; visible under natural light without any special equipment.

3. Easy opreration; a familiar agar plate with E. coli population on it.

We designed “gear” and “clock hand”. The genetic “gear” of the clock, which generates gene expression in response to a stimulus and relays this stimulus to surrounding cells, consists of a positive feedback loop and a Multi-negative feedback system. The “clock hand” to visualize this gene expression is mCherry, a protein visibly red under natural light (Table 1). E. coli possessing this genetic behavior is placed on an agar plate so as to create a circle. Agar plates are relatively inexpensive and easier to use than microfluidic device, which is used to observe the spatiotemporal behavior of gene expression in previous research (Tal Danino et al., 2010 [1]). Agar plate is small and portable while microfluidic device has machines and tubes connected to it. These advantages of agar plates may contribute to the intuitive understanding of clock hand movement, and lead to future applications.

Table 1

The clock device also contains a “control” reporter (amilGFP) for dual-reporter assay and a UV reset device (lambda cI repressor), which are explained later.

Figure 1 Multicellular Analog Clock

Figure 2 The clock device. Pink box genes (luxI and luxR) form the positive feedback loop of AHL production. Blue box genes (tetR, aiiA, and sRNAs) are the suppressors used for negative feedback loops. mCherry is a red reporter protein for visualization of the excitation wave (“clock hand”). amilGFP is the yellow reporter protein expressed constitutively as an internal standard.

The neuron analogy

Our genetic “clock hand” , is similar in many ways to the action potential propagating along nerve cell axons (Fig. 3, Fig.4). Both can be classified as excitation waves which are driven to propagate in one direction by positive and negative feedback loops. Imagine a circle of axon. The propagation of action potential is just like a clock hand movement (Fig. 5). We explain the mechanism of our clock “gear” by analogy with action potential of the nerve cell.

Figure 3 The clock hand moves in one direction; from the past to the future.

Figure 4 One directional propagation of action potential

When stimulated, a pulse of positive membrane potential (action potential) is generated. (Fig. 5). Positive membrane potential opens voltage-gated Na+ channel. Influx of Na+ increases positive membrane potential. This is positive feedback to amplify positive membrane potential. After a few milliseconds, positive membrane potential opens voltage-gated K+ channel. Increased K+ outflux decreases membrane potential. This is negative feedback to decrease positive membrane potential (Fig. 6).

Figure 5 Action potential of nerve cell

Figure 6 Positive and negative feedback loops regulate positive membrane potential to drive the action potential along nerve cell axon.

The positive membrane potential spreads along axon, coupling membrane potential of neighboring region, and causes another rising phase and falling phase. This is observed as action potential propagating along a nerve cell axon.

The feedback loops and coupling creates one-directional movement in nerve cell. Nerve cell and our Clock have the positive feedback followed by negative feedback and coupling in common (Table 2). Thus, as in a nerve cell, the one directional excitation wave may be generated on our Clock (Fig. 7).

Table 2 Action potential and clock hand


Figure. 7

Positive feedback loop

The positive feedback loop of the clock “gear” is composed of luxI (3OC6HSL(AHL) synthetase), the pLux/Tet hybrid promoter, and the luxR activator(Fig. 8).

Fig. 8 Positive feedback loop

This positive feedback loop makes rising phase of gene expression. It works as follows (Fig. 9);

0, LuxR is constitutively expressed in cells.

1, AHL flows in from out of the cell and binds to LuxR.

2, the AHL-LuxR complex binds to the pLux/Tet hybrid promoter and increase the transcription rate of genes including luxI.

3, LuxI synthesizes AHL.

Figure. 9 Positive feedback loop.

This process occurs not only in a single cell. Intercellular positive feedback loops are formed and function as intercellular coupling like a spreading positive membrane potential of a nerve cell axon (Figure 10); AHL is synthesized in a cell and diffuses across cell membrane, and activates transcription in other cells. This cell-cell communication enables intercellular synchronization of gene expression, which drives the one directional movement of the “clock hand”.

Figure 10 Intercellular coupling

Multi-Negative feedback system

Our clock “gear” has 4 negative feedback loops of different mechanisms (Table3), which are formed by tetR, aiiA, anti-mCherry sRNA, and anti-luxI sRNA (Fig. 11). All suppressors are regulated under the pLux/Tet promoter and expressed when stimulated by AHL. This negative feedback loops makes falling phase of gene expression.

Figure 11 Multi-negative feedback system


Table 3 Multi-negative feedback system

TetR binds to pLux/tet and represses transcription of mCherry, luxI, and 4 suppressors including tetR.

AiiA degrades AHL in order to repress transcription indirectly.

The sRNAs might work as a rapid suppressor for two reasons; 1. they repress the translation, which is the final step of prokaryotic gene expression, 2. they are quickly produced since they do not require time for translation of themselves. Both of sRNAs facilitate degradation of mRNA that contains mCherry, luxI, tetR, and aiiA. Anti-mCherry sRNA represses translation of mCherry gene, and anti-luxI does that of luxI gene. Repression of luxI causes decreased production of AHL, while repression of mCherry doesn’t affect AHL.

The differences of mechanisms may lead to various patterns of suppression of various strength and time (Fig. 12). This multi-negative feedback system is a novel one which is not reported in previous papers or past iGEM projects. We designed 20 combinations of these 4 negative feedback loops and sought for the best combination for the Multicellular Analog Clock.

Hasty group reported[1] the system with only one negative feedback loop of AiiA. Their system generated synchronized oscillation. This system shows bursts without any external stimuli. The burst is not suitable for the Analog Clock, because it means inappropriate addition of clock hands. TetR and 2 sRNAs were added in our clock device. These additional suppressors may lead to stronger repression and prevents burst of other clock hands.

Figure 12 Multi-negative feedback system.

The excitation wave

Here, the propagation of excitation wave is explained concluding the discussion above .The positive feedback loop and Multi-negative feedback system drive the “clock hand”, the excitation wave of gene expression. The propagation of excitation wave is generated as follows (Fig. 13);

0. Cells are in the resting state. They are ready to respond to the stimulus by AHL.

1. Cells are activated by AHL synthesized by neighbor cells and express mCherry. These cells are the “clock hand”.

2. The LuxI is also expressed at the same time and synthesize AHL to activate neighbor cells.

3. The supressors are also expressed to make unresponsive state, which prevents “clock hand” go backwards.

Figure 13

Clock hand (visualization for naked eyes)

The clock device has 2 reporter genes for dual-reporter assay, which allows accurate measurement of gene expression intensities.

mCherry is a red reporter protein for the “clock hand”. mCherry expression intensity changes dynamically, being increased by AHL stimulus and decreased by 4 suppressors.

amilGFP is yellow “control” reporter protein for ratiometric measurement with mCherry. amilGFP is constitutively expressed independently from clock “gear”.

Without “control” reporter, background noises (changing agar plate environment surrounding cells, amount of resources for gene expression, cell population density, degradation tag activity, etc.) might obscure the dynamic gene expression of interest (=“clock hand”). Comparing the mCherry expression with amilGFP, you can clearly track the “clock hand” for a long time and will notice the unimportant noises disturbing the clock.

mCherry and amilGFP have strong color, which is visible to naked eyes under natural room light (Fig. 14). You can easily see the gene expression without special equipment.

Also, both of them are fluorescent protein and have different excitation and emission spectra. Measuring fluorescence (e.g. fluorescent microscopy, flow cytometry, …) will be a good way to measure the gene expression accurately. You can measure both fluorescence and color under natural light with one BioBrick device.

amilGFP with LVA degradation tag is a new BioBrick part of our design. This BioBrick part will be used as a good reporter for its strong yellow color, fluorescence, and short lifetime, which enables intuitive understanding and real-time measurement of gene expression at the same time.

LVA degradation tag

C-termini of almost all the proteins in the clock device (luxI, tetR, aiiA, mCherry, and amilGFP) have LVA degradation tag for rapid degradation [2]. Rapid degradation of components of feedback loops may cause rapid change of gene expression, which keeps “clock hand” small. Reporter genes should have short half-lives to visualize real-time changes of gene expression. LuxR has no degradation tag, since clock device needs constant expression of LuxR.

UV reset device

Feedback loops of genetic circuit are not sufficient to generate one directional movement of mCherry “clock hand” along an E. coli circle. If you had only feedback loops, after adding AHL at one point of the circle to start the Clock, you will get two-way movement, which will soon disappear by collision of two “clock hands”.

To turn this two-way movement to one-way, we need to make some part of a circle unresponsive before starting the clock. UV reset device was included in the clock device for this purpose. UV reset device contains UV sensor (UV-susceptible cI repressor and the phage lambda PR promoter and suppressors (Fig. 15). )

Figure 15

UV reset device makes the “unresponsive period” in response to exposure to UV. The detailed mechanism is as follows (Fig. 16)

0, cI repressors are constitutively expressed.

1, UV induces cleavage (inactivation) of cI repressor.

2, phage lambda PR promoter is derepressed.

3, suppressors are expressed, which makes unresponsive period.

Figure 16 UV reset device. UV induces suppressors to make unresponsive state.

UV treatment induces unresponsive period and AHL generate clock hand. One-way movement is generated when UV treatment was next to the point of AHL induction when you start the clock (Fig. 17).

Figure 17 Start of the Multicellular Analog Clock. UV induction is just next to AHL.

UV-susceptible cI repressors (E233K) were used in toggle switch of iGEM MIT 2010. However, MIT 2010[3] made only the composite toggle parts. Thus, we made a new BioBrick part of single UV-susceptible cI repressor (E233K) through site-directed mutagenesis. This standard basic part will be useful for future registry members.

The full system

“Gear”, “Clock hand”, and UV reset device make the full genetic circuit (BBa_K1124100
) (Fig. 18).The circle of E. coli population on agar plate with BBa_K1124100 works as the Multicellular Analog Clock.

Figure 18

Applicaton

In this project, we constructed an analog clock of E.coli for which quorum sensing was utilized. The system is simple because it has only one hand. So other functions can be easily adopted. For example, if particular smell or light is given out every regular time, it becomes an alarm clock. Some past iGEM projects such as MIT2006 and NCBS2009 referred to how to produce scent. In addition, the fine adjustment device could be constructed which is based on the velocity of the hand of it changing by external input. For instance, it is possible to precisely control the clock device by employing promoterAHL which promotes transcription according to the density of AHL and constructing the sequences, such as promoterAHL-luxI and promoterAHL-(tetR, aiiA, sRNAs). While UV reset device resets the clock by decomposition of c1 repressor (E233K), it resets the clock by increase or decrease in AHL.

As another use of clock device, there is a quantity measuring device. The outline is as follows. First, we move the hand by means of the clock device, and then stop the movement of it after a fixed period of time by such as irradiation of UV. As a result, quantity can be visibly measured. For example, we could measure the density of a material by using this device. The material to be measured defines “S”, we make a construction, promoterS-RBS-luxI-d.term-(clock-device)-(UV reset device). PromoterS promotes transcription in response to the existence of S. Then, because the velocity of the hand changes according to the density of S, we could conclude that if it swung longer, the density is higher and that if it swung shorter, the density is lower.(Fig.1)

Fig1. The density of a material measuring device

Not only whole clock device, but also the parts we made for this project constitutive of it, such as amilGFP and UV sensor, could be applied to different purposes. amilGFP with LVA degradation tag gives out strong yellow light under natural light and degrades rapidly. Using these features enables us to measure visually, depending on the flow of time. Also, UV sensor can express any gene in any time by only irradiating UV.

As stated above, the clock device and its parts can be used for various purposes and these applications would also give synthesis biology great progress.

RNA Silencing

Background

Introduction

It is needless to say that gene knockout is important in the study of biology. Genomic knockout by making the target gene mutate requires much labor and time. In eucaryotes, RNAi has contributed to the functional analysis of genes. E. coli, which belongs to procaryotes, does not have the mechanism needed for RNAi and this method is not usually used for E. coli.

But in recent years it has been becoming clear that E. coli has the mechanism of gene silencing by small regulatory RNAs (sRNA) which have complementary sequences with their target mRNAs [1].We used the mechanism for gene knockout by designing synthetic sRNAs and inducing plasmids coding them into bacterial cells. This method can be done by gene introduction and enables us to knock down genes by combining our new BioBrick parts "micC scaffold" and complementary sequences with the target gene [2].

We tested using our BioBrick parts by applying them for the following objectives.

  • Acceleration of Biosynthesis of L-DOPA, a substance utilized for treatment of Parkinson’s disease.
  • Improvement of H2 Productive Capacity of E. coli
  • Suppressor of Multi-Negative Feedback System of Clock Device

System

Mechanism of RNA silencing in Escherichia coli

Escherichia coli have several trans-acting sRNA molecules repressing the expressions of their target genes, such as micC and sgrS and so on. Their mechanism has been becoming clear in recent years. (Fig.1) These sRNA molecules consist of complementary sequences to their target mRNAs and a scaffold sequence that recruites the Hfq protein. Hfq protein, one of the RNA chaperones, facilitates the hybridization of sRNAs to mRNA by their complementary sequences and with RNase E. RNase E causes the degradation of the sRNAs by the target mRNAs. So the mechanism of gene repression by Hfq-dependent sRNAs is composed of the translational repression by their complementary sequences and Hfq proteins and the degradation of the target mRNAs by RNase E [3].

We tried to apply the Hfq protein-dependent RNA silencing for several objectives by establishing the design principle of sRNAs.

Fig.1 Mechanism of expression inhibition by Hfq protein-dependent sRNAs Design and use of synthetic regulatory small RNAs


In order to design Hfq protein-dependent sRNAs simply, we used the following design principle. Our sRNAs are composed of two regions: a target-binding sequence and a scaffold sequence. We made the sRNA generator by combining these with a promoter and a terminator. (Fig.2)

We chose a region of the target sequence that spans from the AUG to nucleotide +21 of the mRNA as the binding site. This was because a previous study suggested that it is effective especially to select the region overlapping the TIR (Translation Initiation Region) of the mRNA [2]. The scaffold sequence recruits the Hfq protein, which facilitates the hybridization of sRNA and target mRNA as well as translational inhibition. Among the scaffold sequences of E. coli, we selected MicC because of the superior repression capability and made the scaffold BioBrick part (BBa_K1124005). In this design, there is an unnecessary sequence, which is caused by using as BioBrick parts, between the promoter and the target-binding sequence.

We proved that the MicC scaffold and our sRNA design with pConst-sRNA(anti-tyrR) functions by qRT-PCR.

Fig.2 Design principle of our synthetic sRNAs


Automatic RNA silencing primer designer

Here we present Automatic RNA silencing primer designer. You can get a pair of primer to generate sRNA sufficient to knockdown the gene of your choice.

Program 1

When you perform PCR with primers designed by Program 1, you will get the PCR product like the diagram below.

You can get promoter-free parts (target binding seq. - micC scaffold - d.term), so you can select any promoters in BioBrick registry.

CDS

Forward Primer for BioBrick

Procedures

1. Input the first 24nt of the CDS of the gene you would like to knockdown.

2. The sequence of forward primer is shown.

3. The sequence of reverse primer is 5-tcaagaactctgtagcaccgcc-3.

4. Perform PCR using primers given by the program. The template must be BBa_K1124112 on the plasmid backbone pSB1C3, pSB1A3, or pSB1AK3.

5. Cut the PCR product by Xba1 and Pst1, and ligate it with backbone.

6. (Alternative to Procedure 5) Cut the PCR product by Xba1 and Pst1, and the promoter part on a plasmid backbone by Spe1 and Pst1. Ligate these digests and you will get the full constructed part that expresses sRNA sufficient to knockdown the gene of your choice.

Program 2

When you perform PCR with primers designed by Program 2 using BBa_K1124123.as the template, you will get the PCR product like the diagram below.

The PCR product is the full constructed part that expresses sRNA sufficient to knockdown the gene of your choice. You never have to digest and ligate the plasmid before E. coli transformation.

CDS

Forward Primer for Inverse PCR

Reverse Primer for Inverse PCR

Procedures

1. Input the first 24nt of the CDS of the gene you would like to knockdown.

2. The sequences of forward primer and the reverse primer are shown.

3. Perform inverse PCR with using primers given by the program. The template is BBa_K1124123. There is no restriction on the plasmid backbone. You must use DNA polymerase with no displacement activity nor 5f¨3fexonuclease activity.

4. The PCR product is a plasmid with nicks. Transform E. coli with the PCR product and the plasmid will expresses sRNA sufficient to knockdown the gene of your choice.

Application

Acceleration of Biosynthesis of L-DOPA

In order to make E. coli produce L-DOPA, we made hpaBC (encoding the enzyme 4-hydroxyphenylacetate 3-hydroxylase) BioBrick parts by cloning it from E. coli BL21 strain. HpaBC catalyzes the hydroxylation of tyrosine and produces L-DOPA (Fig.3a). But the production rate of L-DOPA was low. Then we designed sRNAs knocking down several genes involved in the pathway from glucose to L-Tyr in order to increase the carbon flow to tyrosine synthetic pathway, because the amount is thought to be dependent on that of L-Tyr, the precursor of L-DOPA [4].

We selected tyrR(encoding tyrosine repressor), csrA(encoding carbon-storage regulator, which regulates the expression of enzyme genes involved in glycolysis) and tyrC (phosphoribosylanthranilate isomerase) as the targets [2]. tyrR and csrA have been shown to promote L-Tyr biosynthesis in the previous study.(Fig.3b)

Because these sRNAs are thought to damage the growth of E. coli, we tried several promoters having different strength.

Fig.3a Hydroxilation of tyrosine to L-DOPA by HpaBC

Fig.3b The tyrosine biosynthetic pathway in E. coli and our metabolic engineering strategy


Improvement of H2 Productive Capacity of E. coli

In 2012, we UT-Tokyo made "H2 E. coli", which produces more H2 than usual E. coli. This year we tried to improve its H2 productive capacity further by designing anti-hycA.

E. coli naturally produces hydrogen through a process called mixed acid fermentation (MAF pathway) (Fig.4a). HycA represses the transcription of the proteins of FHL complex, which produces H2 from formate.

Last year we tried to derepress it by introducing plasmids including many decoy binding sites in our project "Inhibition without knockout". This method has several methods. It allows to knockdown by introducing BioBrick parts and does not make scars on the candidate's genome. But it can be used only for DNA binding proteins and the strength cannot be regulated without changing the plasmid.

Though the merit of "Inhibition without knockout" is retained in knockdown with Hfq protein-dependent sRNAs, our new method does not have these demerits.

Fig.4a The function of FHL complex and HycA

Fig.4b The system of "Inhibition without knockout"

By introducing plasmids with many decoy binding sites of the target proteins, we tried to prevent repressors from acting on genomic sites.

Suppressor of Multi-Negative Feedback System of Clock Devise

In our project, Multicellular Analog Clock, we needed a suppressor to be used for the Multi-Negative Feedback System and UV reset devise. Our clock devise uses novel systems which are not seen in previous papers or past iGEM project and it is difficult to say what kind of suppressors is best unless we actually try them. So we designed two kinds of sRNAs that bind to the TIR of mCherry and luxI as the suppressors (anti-mCherry and anti-luxI).

sRNA suppressors will behave differently from AiiA and TetR. sRNAs express and decompose more rapidly than suppresors based on these proteins and act on different targets.

The two sRNAs are act on different molecules within the clock pathway and therefore expected to influence clock behavior differently. Translational inhibition is caused only by sRNAs binding to the TIR of the target gene while degradation by RNase E is caused by any sRNAs binding to the mRNA.

The differences of mechanisms may lead to various patterns of suppression of various strength and time.

References

[1]Aiba, H. (2007). Mechanism of RNA silencing by Hfq-binding small RNAs. Current opinion in microbiology, 10(2), 134-139.

[2]Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013). Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs. Nature biotechnology.

[3]Yoo, S. M., Na, D., & Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocols, 8(9), 1694-1707.

[4]Munoz, A. J., Hernandez-Chavez, G., de Anda, R., Martinez, A., Bolivar, F., & Gosset, G. (2011). Metabolic engineering of Escherichia coli for improving L-3, 4-dihydroxyphenylalanine (L-DOPA) synthesis from glucose. Journal of industrial microbiology & biotechnology, 38(11), 1845-1852.

[5]Maeda, T., Sanchez‐Torres, V., & Wood, T. K. (2008). Metabolic engineering to enhance bacterial hydrogen production. Microbial Biotechnology, 1(1), 30-39.