Template:Team:Bielefeld-Germany/Notebook/Week02

From 2013.igem.org

(Difference between revisions)
m
m
Line 43: Line 43:
                 - Preparing overnight culture ''E. coli'' KRX <br/>
                 - Preparing overnight culture ''E. coli'' KRX <br/>
</p>
</p>
 +
                </div>
                 </div>
                 </div>

Revision as of 19:28, 24 June 2013


Week 2, 14.05.2013, Tuesday

Posted on 10/06/2013

1) Starting with experiment Glycerol dehydrogenase
- Preparing overnight culture ''E. coli'' KRX

Week 2, 15.05.2013, Wednesday

Posted on 10/06/2013

1) Glycerol dehydrogenase
- Using overnight culture ''E. coli'' KRX
- Genome Isolation ''E. coli'' KRX using innuPrep Plasmid MiniKit ''JenaAnalytik''
- Using ''E. coli'' KRX Genome DNA for PCR
- PCR-program:


Table PCR-program
- PCR products are used for agarose gel electrophoresis
Table gel-program
- Agarose gel electrophoresis failed, no bands found

Week 2, 16.05.2013, Thursday

Posted on 10/06/2013

1) Glycerol dehydrogenase
- Using'' E. coli'' KRX Genome DNA for PCR
- PCR-program:
Table PCR-components
Table PCR-program
- PCR products are used for agarose gel electrophoresis
Table gel-program
- Agarose gel electrophoresis worked, see image.
Picture Agarosegel glda_1
- Bands are at expected size of 1050 bp

Week 2, 17.05.2013, Friday

Posted on 10/06/2013

1) Glycerol dehydrogenase
- Repeating agarose gel electrophoresis form 23.05.2013 with more sample volume
- Isolation of bands at 1050 bp using innuPrep Gel Extraction Kit ''JenaAnalytik''
- Measurement of DNA-concentration using nanodrop