10 July 2013

Today, we checked the products of SELEX cycle 8 in order to proceed to cycle 9. We amplified the products using different number of cycles in PCR machine: 10, 12, and 15. It was found that DNA strands amplified using 10 and 12 cycles showed perfect 80bp bands, however DNAs amplified with 15 cycles showed smears in the gel. It means that we can proceed to cycle 9, and we also need to do amplification of SELEX products by setting 10 cycles in PCR machine.

Moreover, we have started preparing for the cloning of aptamers, amplified with PCR, with pGEM Vector. We prepared LB agar plates containing Ampicillin,IPTG and X-Gal, and put the plates at +4degC.