10 September 2013

Today we transformed our aptamers from SELEX 12 using both TOPO TA cloning and pGEM vector cloning (See protocols for details).

In addition, we repeted colony PCR of streptavidin and checked it with agarose gel electrophoresis, which showed the appropriate size of this part.

We amplified our DNA from selex 12 again, and when checking its size on gel showed that it had some smeared bands, i.e. contamination, we extracted the clear 80bp band of DNA from the gel, purified it, and after purification, it was found that DNA was pure and had 80bp size.