11 September 2013

Today we checked our results of cloning and transformation. There were no colonies for TOPO TA, probably, because the cells that were used were not competent. However, there were some colonies growing on ampicillin plates for pGEM vector. We isolated a single colony, but it in SOC medium for 1 hour and then plated again on LB agar/ampicillin (50ug/ml) plate.

We repeated TOPO TA cloning using new competent cells for transformation and plated them on LB agar/ampicillin plates plus the control LB agar plate.