12 July 2013

From 2013.igem.org

No growth was observed on the plates made yesterday. Possible reasons:

- transformation did not occur
- cells used were not competent
To test the competence of the cell we cultivated SA, BC and PC samples and cells sample alone into four separate tubes with LB broth and left for overnight incubation.
If no growth will be observed tomorrow then we will have to use different competent cells