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From 2013.igem.org

Single digest

• Digesting RFP plasmid again using a single restriction enzyme- XbaI
• Digesting DNA samples 1-6
• 200ng of DNA is run
• Volumes for digest:

Sample nr	1	2	3	4	5	6
DNA	0.7	0.7	0.8	1.3	1.2	2.6
Buffer	2	2	2	2	2	2
Water	16.3	16.3	16.2	15.7	15.8	14.4
XbaI	1	1	1	1	1	1

• Buffer used was cutsmart
• Incubate samples at 37C for 1h
• 2ul of orange G dye was added to each samples
• Samples were then frozen overnight to run on gel on Monday