19 August 2013

1. No colonies grew on yesterday plates with antibiotics. It may mean that transformation did not occur.

2. Very weak colonies have grown on control LB agar plates.

3. Transformed cells containing control DNA from TOPO TA kit were seeded on LB agar plates from liquid LB broth and were put for incubation at 37degC overnight.

4. E. Coli HB 101 K cells were seeded on LB agar plates and LB broth, and were put for incubation overnight (37degC, 100rpm).