2 September 2013

All cultures of yeast left for prorulation 5 days ago were not appropriate for further manipulations. After observing each sample microscopically, we could see either non-meiotic yeast cells or nothing. Therefore the procedure was done from the beginning. The loopful of culture from glycerol stock was innoculated into each YPD containing tube and left for overnight incubation.

SDS PAGE for biotynilated pritein was performed. The results showed that the protein was less than 180kDa, which may indicate the fact that it somehow divided into smaller fragments. To recheck these results, DOT ELISA will be performed tomorrow