6 June 2013

From 2013.igem.org

Electromobility shift assay (EMSA) was performed in order to check the CEA-DNA aptamer interaction. For this experiment aptamers from SELEX 5 and 7 were used. Visualization was done with ethidium bromide post staining, however no bands were observed. The possible reasons for this are either very low concentrations of protein and DNAs were used or certain errors were done in preparing the required reagents.