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Exeter/12 August 2013
From 2013.igem.org
Mini Preps of liquid cultures that worked
NanoDrop
| Culture | NanoDrop concentration (ng/ul) | ul to get 250ng |
|---|---|---|
| AMP plasmid | 60.0 | 4.17 |
| Yellow (K592010) | 49.1 | 5.09 |
| RBS + cph8 | 81.2 | 3.08 |
| Terminators | 27.9 | 8.96 |
| RFP control | 51.3 | 4.87 |
| RBS + yellow (S05058) | 56.7 | 4.41 |
| ompC | 76.7 | 3.20 |
| Promoter + RBS | 62.6 | 3.99 |
| Magenta | 368.1 | 0.67 |
| Magenta diluted | 35.7 | 7.0 |
| Culture | ul to get 250ng | 16ul - answer |
|---|---|---|
| AMP plasmid | 4.17 | 11.83 |
| Yellow (K592010) | 5.09 | 10.91 |
| B0015 | 8.96 | 7.04 |
| RFP | 4.87 | 11.13 |
| RBS + yellow (S05058) | 4.41 | 11.59 |
| Magenta diluted | 7.0 | 9.0 |
| Culture | DNA (pigment) + DNA (term) + DNA plasmid | How much DNA is in there | +T4 | Water |
|---|---|---|---|---|
| Magenta | 0.648 + 0.12 + 2.0 | 2.768 | 4.268 | 5.732 |
| Yellow (K592010) | 0.664 + 0.12 + 2.0 | 2.784 | 4.284 | 5.716 |
| Yellow (S05058) | 0.680 + 0.12 + 2.0 | 2.80 | 4.30 | 5.70 |
| RFP | 0.992 + 0.12 + 2.0 | 3.112 | 4.612 | 5.388 |
Gel of digest
| Lane | Content |
|---|---|
| 1 | 1kb Gene Ruler |
| 2 | Yellow (S05058) |
| 3 | Magenta |
| 4 | Yellow (K592010) |
| 5 | RFP |
| 6 | AMP plasmid |
| 7 | B0015 |
| 8 | 1kb Gene Ruler |
Unfortunately lanes 6 and 7 appear to have not worked. This may be due to human error, or a very low concentration of DNA being pipetted into the wells; the amount is insufficient to show a band.
Take me back to the notebook.
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