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Exeter/12 September 2013
From 2013.igem.org
Optical Absorption of liquid cultures
Using the liquid cultures that were made yesterday afternoon, today we used a plate reader to measure the absorbance of the cyan and magenta cultures. Cyan was measured every 4 hours as the colour appears to be developing much slower than the others, but the magenta was measured every two hours.
| Liquid culture | Absorbance at 10:00am | Absorbance at 12:00pm | Absorbance at 14:00pm | Absorbance at 16:00pm |
|---|---|---|---|---|
| Cyan no.2 | 0.602 | - | ? | - |
| Cyan no.3 | 0.817 | - | ? | - |
| Cyan V.2 | 0.894 | - | ? | - |
| Cyan V.4 | 0.740 | - | ? | - |
| G block 11 | 0.542 | 0.519 | ? | ? |
| G block 7 | 1.023 | 1.120 | ? | ? |
Transformations
In the afternoon we transformed the ligations from earlier in the week. This was also done on Tuesday, however, we now have fresh competent cells that we used instead.
Take me back to the notebook.
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