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Exeter/19 July 2013
From 2013.igem.org
Transformations
We are transforming...
- New Part 1 - CcaS (green light sensor) + terminator
- New Part 2 - (promoter, RBS and FixJ) + terminator
- New Part 3 - Yellow pigment + terminator
- New Part 4 - Magenta pigment + terminator
- New Part 7 - YF1 (blue light sensor) + terminator
- New Part 8 - FixJ + terminator
- Ligation control (RFP)
- Retry of OmpR (BBa_K098011) from Kit Plate (failed on 16/7/13)
- Retry of lambda repressor system (BBa_Q04510) from Kit Plate (failed on 16/7/13)
- Different version of RBS + cph8 (red light sensor) from Kit Plate
(Monday morning, results of transformations)
| Part | Number of colonies |
|---|---|
| Different version of RBS + cph8 | 23 |
| OmpR | 120 |
| Lambda repressor system | 20 countable, other colonies visible but too small to numerate |
| RFP control | 75 |
| New Part 1 | 78 |
| New Part 2 | 2 |
| New Part 3 | 12 |
| New Part 4 | 0 |
| New Part 7 | 6 |
| New Part 8 | 1 |
| Ligation Control | colonies present, too small to count, but approx. 100 |
Obviously, these results aren't fantastic. They are far better than our first attempt at digestions/ligations/transformations (we actually have colonies this time!) but until we can make liquid cultures and run some gels, we can't really know if they've worked.
For this reason, we have been investigating other assembly methods. We have a few ideas on the go, but if you have any recommendations or tips, we'd love to hear them! You can always reach us through igem@ex.ac.uk.
Take me back to the notebook.
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