Exeter/29 August 2013
From 2013.igem.org
Minipreps
We miniprepped:
- 1 + 4
- [http://parts.igem.org/Part:BBa_K592011 K592011] B
- [http://parts.igem.org/Part:BBa_K864404 K864404]
- Cph8 #3
- 2 + 4
- [http://parts.igem.org/Part:BBa_K592022 K592022] B
- ENvZ no antibiotic
- ENvZ
- Cph8 #5
The liquid cultures of [http://parts.igem.org/Part:BBa_K592011 K592011] A and [http://parts.igem.org/Part:BBa_K592022 K592022] A did not grow.
NanoDrop
The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.
- K592011 B - 164.2 ng/ul
- B0015 - 37.7 ng/ul
- OmpR prom - 50.7 ng/ul
- RBS - 49.1 ng/ul
Two different RFP controls were nanodropped
- RFP control - 61.7 ng/ul
- RFP control - 75.9 ng/ul
The first RFP control was used in the digestion of K592011 ans B0015. The second RFP control was used in the digestion of OmpR and RBS.
Digestion
Part | K592011 (Part A) | B0015 (Part B) | AMP Plasmid | RFP (Control) |
---|---|---|---|---|
DNA | 1.52 ul | 6.63 ul | 10.0 ul | 4.05 ul |
Non-nuclease Water | 14.48 ul | 9.37 ul | 5.5 ul | 11.95 ul |
NEB Buffer | 2.5 ul | 2.5 ul | 2.5 ul | 2.5 ul |
BSA | 0.5 ul | 0.5 ul | 0.5 ul | 0.5 ul |
EcoRI | 0.5 ul | - | 0.5 ul | 0.5 ul |
SpeI | 0.5 | - | - | - |
XbaI | - | 0.5 ul | - | - |
PstI | - | 0.5 ul | 0.5 ul | 0.5 ul |
DpnI | - | - | 0.5 ul | - |
Part | OmpR (Part A) | RBS (Part B) | AMP Plasmid | RFP (Control) |
---|---|---|---|---|
DNA | 4.93 ul | 5.09 ul | 10.0 ul | 3.30 ul |
Non-nuclease Water | 11.07 ul | 10.91 ul | 5.5 ul | 12.70 ul |
NEB Buffer | 2.5 ul | 2.5 ul | 2.5 ul | 2.5 ul |
BSA | 0.5 ul | 0.5 ul | 0.5 ul | 0.5 ul |
EcoRI | 0.5 ul | - | 0.5 ul | 0.5 ul |
SpeI | 0.5 | - | - | - |
XbaI | - | 0.5 ul | - | - |
PstI | - | 0.5 ul | 0.5 ul | 0.5 ul |
DpnI | - | - | 0.5 ul | - |
Take me back to the notebook.