Exeter/3 September 2013
From 2013.igem.org
Results of liquid cultures from yesterday
gBlocks
gBlock | Colour |
---|---|
1 | No |
2 | No |
3 | Vibrant pink |
4 | No |
5 | Vibrant pink |
6 | No |
7 | Peach |
8 | No |
9 | Pink - not so vibrant |
10 | Vibrant pink |
11 | Vibrant pink |
12 | Pink - not so vibrant |
13 | Pink - not so vibrant |
14 | No |
15 | No |
Part | Did it work? |
---|---|
ompF + RBS A | ✓ |
ompF + RBS B | ✓ |
ompF + RBS C | ✓ |
ompF + RBS D | ✓ |
K592022 A | ✗ |
K592022 B | ✓ |
K592011 A | ✗ |
K592011 B | ✓ |
K864404 | ✓ |
Miniprepped
- ompC + RBS A
- ompC + RBS B
- ompC + RBS C
- ompC + RBS D
- [http://parts.igem.org/Part:BBa_K592011 K592011 B]
- gblocks 1 to 15
NanoDrop from miniprep
Part | Concentration (ng/ul) |
---|---|
gBlock 1 | 27.4 |
gBlock 2 | 28.6 |
gBlock 3 | 101.2 |
gBlock 4 | 37.2 |
gBlock 5 | 168.6 |
gBlock 6 | 21.3 |
gBlock 7 | 153.7 |
gBlock 8 | 30.8 |
gBlock 9 | 247.1 |
gBlock 10 | 183.4 |
gBlock 11 | 197.4 |
gBlock 12 | 105.1 |
gBlock 13 | 126.6 |
gBlock 14 | 172.5 |
gBlock 15 | 87.0 |
ompF + RBS A | 41.2 |
ompF + RBS B | 57.8 |
ompF + RBS C | 74.2 |
ompF + RBS D | 46.7 |
K592011 B | 159.0 |
Take me back to the notebook.