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Exeter/5 August 2013
From 2013.igem.org
Glycerol stocks
We made up 3 replicates of:
[http://parts.igem.org/Part:BBa_K592018 K592018]
[http://parts.igem.org/Part:BBa_S05058 S05058]
[http://parts.igem.org/Part:BBa_B0015 B0015]
Nano Drop
| Culture | NanoDrop concentration (ng/ul) | size |
|---|---|---|
| K592018 | 160.7 | 2526bp |
| S05058 | 118.3 | 717bp |
| RFP | 96.1 | 1047bp |
| B0015 | 76.3 | 129bp |
Digestion
| Part | DNA (ul) | dH20 (ul) | Buffer (ul) | BSA (ul) | Enzyme 1 (ul) | Enzyme 2 (ul) | Enzyme 3 (ul) |
|---|---|---|---|---|---|---|---|
| K592018 | 1.6 | 14.4 | 2.5 | 0.5 | EcoRI 0.5 | SpeI 0.5 | - |
| S05058 | 2.1 | 13.9 | 2.5 | 0.5 | EcoRI 0.5 | SpeI 0.5 | - |
| B0015 | 3.3 | 12.7 | 2.5 | 0.5 | XbaI 0.5 | PstI 0.5 | - |
| AMP plasmid | 10 | 6 | 2.5 | 0.5 | EcoRI 0.5 | PstI 0.5 | Dpnl 0.5 |
| RFP | 2.6 | 13.4 | 2.5 | 0.5 | EcoRI 0.5 | PstI 0.5 | - |
Ligation
Two methods using 'standard' amounts of each digest and using equimolar amounts.
Take me back to the notebook.
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